In order to explore the macrofungal resources and distribution system in the Irtysh River Basin,this study used the interview method,sampling method and line transect method to conduct surveys in the Irtysh River Basin from June to August 2022 and July to August 2023 respectively.The macrofungal resources were investigated and monitored in the main cultivated land,natural pastures,forest areas and edible fungus planting areas.The study collected a total of 136 macrofungal samples at 692 inspection points.It was identified that there were 2 phyla,2 classes and 6 macrofungal species in the area.There are 31 species in 14 orders,24 genera and 14 families.Among them,the genus Coprinus is the most abundant,with 4 species accounting for 12.90%of the total number of species;the genus Panaeolus is second,with 3 species accounting for 9.68%of the total species;Pleurotus and Cortinarius have 2 species accounting for 6.45%of the total number of species,and the remaining 20 genera only have 1 species.The spatial distribution characteristics of macrofungi in the Irtysh River Basin show that they are more densely distributed in woodlands and grasslands along the river basin,while there are less distribution in construction lands,reflecting the environmental and ecological indicator function of macrofungi.In addition,monitoring of habitat factors of large fungal fruiting bodies shows that the distribution of large fungal fruiting bodies is related to environmental factors such as temperature and humidity.Among them,Stropharia rugosoannulata is extremely resistant to environmental stress and can be used as a potential edible cultivated fungi.This study provides back ground resource data for subsequent edible fungi industry breeding,ecological protection and restoration,and improvement of ecosystem services in the region.