肥大细胞糜蛋白酶在瘢痕疙瘩组织的活性表达
The expression of mast cells chymase activity in keloid tissue
徐涛 1董祥林1
作者信息
- 1. 新疆医科大学第一附属医院烧伤整形科，乌鲁木齐 830054
- 折叠
摘要
目的：探讨肥大细胞糜蛋白酶在人瘢痕疙瘩组织中的表达活性。方法采用免疫组织化学法检测瘢痕疙瘩组织和正常皮肤中肥大细胞的数量和糜蛋白酶的表达，并以 RT-PCR 和放免法检测瘢痕疙瘩和正常皮肤组织中糜蛋白酶 mRNA 表达活性变化。结果瘢痕疙瘩组织中肥大细胞数量与糜蛋白酶阳性表达评分为（10±0．25）个／HP、（6．2±0．23）mm2，明显高于正常皮肤组织的（4±0．22）个／HP、（2．5±0．12）mm2（P ＜0．01）；RT-PCR结果显示瘢痕疙瘩组织中肥大细胞 mRNA 和糜蛋白酶 mRNA 表达水平较正常皮肤组织明显增高。结论肥大细胞糜蛋白酶存在于瘢痕疙瘩中且活性表达明显高于正常皮肤组织。
Abstract
Objective To explore the expressed activity of mast cells chymase exist in human keloid tissues. Methods The number of mast cell and chymase expression in keloid and normal skin were assessed by im-munohistochemical methods.Quantitative real-time PCR and Radio immunity method were conducted to measure the change of mRNA expressed activity of chymase in keloid and normal skin tissues.Results Com-pared with normal skin tissue,the number of mast cells and chymase expression were higher in keloid tissues [(10 ±0.25)/hp vs (4±0.22)/hp,and (6.2±0.23)mm2 vs (2.5±0.12)mm2 ,P <0.01)].The results of RT-PCR showed mast cell mRNA expression levels and chymase mRNA level were also significantly higher in keloid tissues than those of normal skin tissues (P <0.05).Conclusion The chymase of mast cells exists in keloids and the expressed activity of chymase is statistically higher than those of normal skin tissues.
关键词
肥大细胞糜蛋白酶/瘢痕疙瘩/活性表达Key words
keloid/mast cells chymase/activity引用本文复制引用
基金项目
新疆医科大学第一附属医院青年科研基金(2012QN02)
出版年
2015
NETL
NSTL
维普
万方数据