Identification and breeding of the H2-Eb1+H2-Ab1 double knockout mice
Objective To feed,breed and identify the H2-Eb1+H2-Ab1 double gene knockout mice,in or-der to get the double homozygote type of H2-Eb1 -/- + H2-Ab1 -/- mice and double wild type of H2-Eb1 +/+ +H2-Ab1 +/+ mice,thereby laying the foundation for the study of the H2-Eb1and the H2-Ab1. Methods After the adaptive feeding of the knockout mice for 2 weeks,the female heterozygote type mice and the male heterozygous type mice were mated.The DNA of rat tail was extracted;the genotyping was determined using PCR method.The wild type mice and the homozygous type will be used for the following biological function research;the heterozygous type mice will be used for reproduction and survive.Results After successful reproduction,There were 20 double homozygote type of H2-Eb1 -/- + H2-Ab1 -/- (20％), 26 double wild type of H2-Eb1 +/+ +H2-Ab1 +/+ (26％),54 of unidentified the genes (54％).the homozy-gous type double knockout mice of H2-Eb1+H2-Ab1 were successfully established,and the breeding,re-production and appraisal method were done expectedly.Conclusion Mating of the male and female of het-erozygous type of double knockout mice of H2-Eb1 + H2-Ab1,not only can give birth to he homozygous type double knockout mice of H2-Eb1 + H2-Ab1,but also good for the reproduction and breeding long-term.
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NSTL
万方数据
维普
