紫草素联合半导体激光预防釉质脱矿的体外研究
Study on prevention of enamel demineralization by Shikonin combined with semiconductor laser in virto
范文献 1范译丹 2赵今 1李新尚3
作者信息
- 1. 新疆医科大学第一附属医院(附属口腔医院)牙体牙髓科,乌鲁木齐 830054
- 2. 新疆医科大学第一附属医院(附属口腔医院)口腔修复科,乌鲁木齐 830054
- 3. 新疆医科大学第一附属医院(附属口腔医院)口腔急诊综合科,乌鲁木齐 830054
- 折叠
摘要
目的 研究紫草素联合半导体激光对牙釉质表面脱矿的抑制作用.方法 收集2021年12月-2022年7月在新疆医科大学第一附属医院(附属口腔医院)就诊的120位15～20岁因正畸减数拔牙的新鲜前磨牙或第三磨牙90颗,用低速金刚石锯将其切割制备成5 mm×5 mm× 3 mm釉质块,选择硬度值在310～350 HV范围内的牙釉质块样本.随机分类法将其分为对照组、紫草素组、激光组、激光联合氟化钠组、氟化钠组、激光联合紫草素组,每组15颗.每组进行3 d的脱矿实验(期间每隔12 h各组按条件给予干预)后,采用显微硬度仪测定各组表面硬度;采用原子力显微镜测定各组脱矿深度、表面粗糙度和表面形貌;采用X射线能谱仪测定各组表面形貌和表面元素变化.结果 与对照组、激光组、紫草素组比较,激光联合紫草素组显微硬度值增大,差异有统计学意义(P＜0.05);与激光联合氟化钠组比较,激光联合紫草素组显微硬度值减小,差异有统计学意义(P＜0.05);与氟化钠组比较,激光联合紫草素组显微硬度值差异无统计学意义(P＞0.05).与对照组、激光组、紫草素组比较,激光联合紫草素组表面粗糙度和脱矿深度减小,差异有统计学意义(P＜0.05);与激光联合NaF组比较,激光联合紫草素组表面粗糙度和脱矿深度增大,差异有统计学意义(P＜0.05);NaF组比较,激光联合紫草素组表面粗糙度和脱矿深度差异无统计学意义(P＞0.05).与对照组比较,激光联合紫草素组表面钙磷元素摩尔比值增大,差异有统计学意义(P＜0.05);与激光联合NaF组、NaF组比较,激光联合紫草素组表面钙磷元素摩尔比值减小,差异有统计学意义(P＜0.05);与激光组、紫草素组比较,激光联合紫草素组表面钙磷元素摩尔比值差异无统计学意义(P＞0.05).结论 紫草素联合半导体激光对牙釉质表面脱矿有明显的抑制作用.
Abstract
Objective To research the effects of Shikonin combined with semiconductor laser on enamel sur-face demineralization by in vitro experiments.Methods In this experiment,90 fresh premolars or third molars of 120 patients aged 15-20 years old attending the hospital for orthodontic minus extractions from December 2021 to July 2022 were uniformly collected,and the patients were cut and prepared into 5 mmX 5 mm×3 mm enamel blocks using a low-speed diamond saw,and hardness values in the range of 310～350 HV were selected for the enamel block samples.Demineralization experiment:the patients were divided into control no treatment group,Shikonin group,laser group,laser+NaF group,NaF group and laser+Shikonin group according to random classification method with 15 samples in each group.Each group was intervened by different methods for 3 days of demineralization experiments(during which interventions were made every 12 hours according to each subgroup),and then the surface hardness of the samples was detected by microhardness tester;the surface demineralization depth and surface roughness,surface mor-phology of the samples were evaluated by atomic force microscopy(AFM);and the surface morphology and surface elemental changes of the samples were studied and analyzed by X-ray energy spectrometry(EDS).Results Compared with the control group,laser group,and shikonin group,the microhardness value of the laser combined with shikonin group was increased,and the difference was statistically signifi-cant(P＜0.05).Compared with the laser combined with sodium fluoride group,the microhardness value of the laser combined with shikonin group was decreased,and the difference was statistically significant(P＜0.05).Compared with the sodium fluoride group,there was no statistically significant difference in mi-crohardness values between the laser combined with shikonin group(P＞0.05).Compared with the con-trol group,laser group,and shikonin group,the surface roughness and demineralization depth of the laser combined with shikonin group was decreased,and the difference was statistically significant(P＜0.05).Compared with the laser combined with NaF group,the laser combined with shikonin group showed an in-crease in surface roughness and demineralization depth,and the difference was statistically significant(P＜0.05).Compared with the NaF group,there was no statistically significant difference in surface rough-ness and demineralization depth between the laser combined with shikonin group(P＞0.05).Compared with the control group,the molar ratio of surface calcium and phosphorus elements in the laser combined with purpurin group was increased,and the difference was statistically significant(P＜0.05).Compared with the laser combined with NaF group and NaF group,the molar ratio of surface calcium and phosphor-us elements in the laser combined with shikonin group was decreased,and the difference was statistically significant(P＜0.05).Compared with the laser group and the shikonin group,there was no statistically significant difference in the molar ratio of surface calcium and phosphorus elements between the laser com-bined with shikonin group(P＞0.05).Conclusion Shikonin combined with semiconductor laser has a significant inhibitory effect on tooth enamel surface demineralization.
关键词
牙釉质/半导体激光/紫草素/脱矿/原子力显微镜/体外研究/微观形貌Key words
dental enamel/semiconductor laser/shikonin/demineralization/atomic force microscopy/in vitro study/microstructure引用本文复制引用
基金项目
中华口腔医学会西部临床科研基金(CSA-W2021-02)
出版年
2023
NETL
NSTL
维普
万方数据