Objective To investigate the expression and clinical significance of follicular helper T cells(Tfh)and follicular regulatory T cells(Tfr)in peripheral blood of patients with acute myeloid leukemia(AML).Methods Sixty newly diagnosed AML patients and forty healthy subjects were selected,the pro-portion of Tfh and Tfr cells was detected by flow cytometry,and the expression levels of serum cytokines IL-10,TGF-p1 and IL-21 were detected by ELISA.The expression levels of transcription factors BCL-6 and Blimp-1 in Tfh and Tfr cells were detected by WB.The proportion of cells and the expression of tran-scription factors and related cytokines were compared between the 2 groups.To analyze the association be-tween Tfr,Tfh/Tfr ratio,cytokines IL-10 and TGF-β1 levels and poor prognosis in AML patients.Res-ults Compared with healthy control group,the proportion of Tfr cells,serum IL-10 and TGF-β1 levels were increased in AML group,and Tfh/Tfr ratio and Blimp-1 level were decreased,with statistical signifi-cance(all P＜0.05).The proportion of Tfh cells,BCL-6 and serum IL-21 levels were slightly increased,with no statistical significance(P＞0.05).Compared with the other 2 groups,the proportion of Tfr cells,IL-10 and TGF-β1 in the poor prognosis group were significantly increased,and the ratio of Tfh/Tfr was significantly decreased(all P＜0.05).Tfr cell proportion,IL-10 and TGF-β1 levels in the medium prog-nosis group were slightly higher than those in the good prognosis group(P＜0.05),Tfh/Tfr ratio was significantly lower than that in the good prognosis group(P＜0.05).Pearson correlation analysis showed that Tfr cell proportion was positively correlated with serum IL-10 and TGF-β1 levels(r=0.706,0.619,both P＜0.000 1),and Tfh/Tfr ratio was negatively correlated with IL-10 and TGF-β1 levels(r=-0.454,-0.446,P＜0.001).Conclusion Tfr cells and related cytokines are abnormally high expressed in AML patients,and there is an imbalance of Tfh/Tfr ratio,and this phenomenon is more obvious in AML patients with poor prognosis.
follicular helper T cellsfollicular regulatory T cellscytokinesacute myeloid leukemia
维普
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