Experimental study of different doses of adenine to establish a rat model of chronic renal failure with spleen-kidney Yang-deficiency
Objective To compare the effects of different doses of adenine gavage on the general state,bio-chemical indicators and renal pathological tissue of the model rats,and determine the optimal dosage of ad-enine to prepare the rat model of chronic kidney disease(CRF)with spleen-kidney-Yang deficiency.Meth-ods 24 SD male rats were randomly divided into normal group and model group,and the model group rats were divided into M1 group,M2 group and M3 group,with 6 rats in each group.In the normal group giv-ing the same amount of normal saline gavage,M1 group was gavaged with adenine 150 mg·kg-1·d-1,M2 group was gavaged with adenine 200 mg·kg-1·d-1,and M3 group was gavaged with adenine 300 mg·kg-1·d-1.After 3 weeks of serum creatinine(Scr),blood urea nitrogen(BUN),and 24-hour urinary protein quantification(24 h-UTP)levels were detected before and every week after molding,and renal pathological tissues were taken after 3 weeks to model the renal histopathology of rats by hematoxy-eosin(HE)and Masson staining.Results Compared with normal group of the rats,model group of the rats showed varying degrees of mental depression,decreased mobility,hunched back,sparse and dull hair,pale and cool ear and toe edges,frequent urination and loose stools.Compared with normal group of rats,each model group showed varying degrees of weight reduction(P＜0.05).At the second week of modeling,compared with M1 group of the rats,there were statistically significant differences in weight between the M2 and M3 groups(P＜0.05).Compared with normal group of the rats,24-hour urine out-put of each model group rats was significantly increased(P＜0.05),and the levels of Scr,BUN,and 24-hour UTP were all increased(P＜0.05),with M3 group rats showing the most significant increase in va-rious indicators.The differences in Scr,BUN and 24 h UTP among the different model groups of the rats were statistically significant(P＜0.05).The results of renal pathological staining showed that the renal tissue of the rats in each model group changed to varying degrees.Microscopically,the glomerular mor-phology was basically complete and some glomerular structures were destroyed.Some proximal renal tu-bules had serious vacuolar degeneration,epithelial atrophy,some renal tubules had dilated lumen,and there were many red blood cells in the lumen.With the increasing of adenine perfusion dose,a large num-ber of yellow-green crystalline precipitates can be seen in glomerulus,and there were inflammatory cell in-filtration and fibrous tissue hyperplasia in renal interstitial.Conclusion Adenine gavage can successfully establish an animal model of spleen-kidney Yang deficiency chronic renal failure,among which the 200 mg·kg-1·d-1 gavage group is closer to the development process of clinical spleen-kidney-Yang defi-ciency chronic renal failure,and the mortality rate is low,which is in line with the physiological and path-ological characteristics of spleen-kidney-Yang deficiency chronic renal failure.
chronic renal failurespleen and kidney Yang deficiencyadenineanimal models
万方数据
维普
