Objective To study the absorption characteristics of allicin ointment on isolated rat skin.Meth-ods A transdermal model of intact dorsal skin and exfoliated skin of the rats was established using a Franz diffusion cell.The indicator content of allicin in the transdermal absorption solution of allicin ointment was determined using ultra-high performance liquid chromatography mass spectrometry tandem mass spec-trometry(UPLC-MS-MS).The in vitro transdermal absorption characteristics of allicin ointment were de-termined,and the cumulative transmittance and permeation rate of allicin was evaluated.Results The lin-ear relationship between the in vitro transdermal penetration of allicin Q within 5 hours and time t was good,which conformed to Higuchi kinetic process.The regression correlation coefficients(r)were all a-bove 0.99.And the transdermal rate of intact skin J was 12.306 μg·cm-2·h-1,J of exfoliated skin was 58.353 μg·cm-2·h-1,the J of exfoliated skin was much higher than that of intact skin.Conclusion The effec-tive ingredients of allicin ointment can penetrate through the skin and are mainly absorbed in the prototype form.The stratum corneum is an important factor affecting the penetration of allicin ointment through the skin.
关键词
大蒜/大蒜辣素/软膏/超高效液相色谱-质谱-质谱串联/透皮吸收/渗透速率
Key words
Allium sativum L/allicin/ointment/ultra-high performance liquid chromatography mass spec-trometry tandem mass spectrometry(UPLC-MS-MS)/transdermal absorption/permeation rate
维普
万方数据