Effect of porphyromonas gingivalis(P.g)upregulation of CCL20 expression on oral squamous cell phenotype
Objective To investigate the effects of porphyromonas gingivalis(P.g)on the proliferation,migration and invasion of human oral squamous cell carcinoma(OSCC)cells by inducing the expression of CCL20,and to further clarify the effect of CCL20 on the phenotype of OSCC cells.Methods The clinical data,peripheral blood and OSCC tissue samples of 44 patients who were admitted to the hospital from 2021 to 2023 were collected,and the peripheral blood of 22 healthy volunteers were collected as control group.The expression of CCL20 in OSCC tissue and serum was detected by immunohistochemistry and ELISA.A cell bacterial co culture model was established by culturing human oral squamous cell carcinoma CAL27 and SCC25 cells in vitro.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and enzyme-linked immunosorbent assay(ELISA)were used to detect the expression of CCL20 in CAL27,SCC25 cells and P.g group.The effects of P.g bacteria and CCL20 on the proliferation,migra-tion and invasion of OSCC cells were examined by CCK-8,cell scratch and transwell tests.Results The expression of CCL20 in OSCC tissues were significantly higher than that in paracancer tissues(P<0.05).Compared with control group,the mRNA and protein levels of CCL20 in OSCC cells CAL27 and SCC25 infected with P.g bacteria were significantly up-regulated(P<0.05).The proliferation,migration and invasion ability of CAL27 and SCC25 cells were all enhanced under P.g infection(P<0.05),while the proliferation,migration and invasion ability of P.g on OSCC cells was weakened after blocking CCL20 ex-pression(P<0.05).Conclusion P.g bacteria can promote the proliferation,migration and invasion of OSCC by up-regulating the expression of CCL20 in oral squamous cell.
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