Rapid deposition of tacrolimus concentration in serum by surface enhanced Raman spectroscopy based on KlariteTM chip
Objective To establish a method for rapid determination of tacrolimus concentration in serum u-sing KlariteTM chip surface enhanced Raman spectroscopy technology.Methods The Raman spectra and characteristic peaks of tacrolimus capsule powder were determined using KlariteTM chip surface enhanced Raman spectroscopy technology,and vibration mode assignment analysis was performed on each peak.The detection limits of tacrolimus in aqueous and PBS buffer solutions on the chip surface were determined using drop coating deposition Raman(DCDR)technology to clarify the efficacy of the 5th generation Klar-iteTM chip in determining tacrolimus concentration.Results The Raman spectra and characteristic peaks of tacrolimus capsule powder were determined using KlariteTM chip surface enhanced Raman spectroscopy technology,and vibration mode assignment analysis was performed on each peak.The detection limits of tacrolimus in aqueous and PBS buffer solutions on the chip surface were determined using DCDR technolo-gy to clarify the efficacy of the 5th generation KlariteTM chip in determining tacrolimus concentration.Ra-man spectroscopy showed that tacrolimus produced sharp peaks at 354～396,474,851～917 and 1 088 cm-1,and a clear broad peak at 1 265～1 475 cm-1.The detection limits of tacrolimus in distilled water and PBS solution were 0.14,respectively μg/mL and 2.5 μg/mL,with quantification limits of 0.47 μg/mL and 8.3 μg/mL.The regression equation for quantitative detection of tacrolimus in distilled water was Y= 52.86X+596.5,and the concentration of tacrolimus ranges from 0.1 to 50.0 μg.The linear relationship was good within the range of g/mL,with a correlation coefficient of 0.890 5.The regression equation for quantitative detection of tacrolimus in PBS solution was Y=19.54X+537.8,and the concentration of ta-crolimus ranges from 5.0 to 50.0 μg,with a correlation coefficient of 0.217 6.In a human serum solution diluted in proportion(1∶4,v/v),Raman spectral characteristic peaks of tacrolimus can be detected main-ly distributed at 352,488,855,1 135,1 213 and 1 456 cm-1,with significantly reducing peak intensities and varying degrees of frequency shift.Conclusion This detection method has high sensitivity,simple opera-tion,fast and convenient operation,and itis suitable for clinical monitoring of tacrolimus blood concentration.
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