Experimental study of effect of Xinjiang Mori Cortexextract on maincariogenic bacteriabiofilm
Objective To explore the effects of Xinjiang Mori Cortex extract on cariogenic virulence factors,such as acid and sugar production of major cariogenic bacteria in biofilm state,as well as biofilm activity.Methods Prepared suspensions of 3 single bacterial strains,namely Streptococcus mutans,streptococcus sanguineum,and streptococcus mucilaginosus,and divided them into a negative control group[without adding Xinjiang Mori Cortex extractor chlorhexidine(CHX)]and positive control group(with 0.05％CHX added).The experimental group(with different concentrations of Xinjiang Mori Cortex extract add-ed)measured the effect of Xinjiang Mori Cortex extract on the acid production ability of 3 cariogenic bacte-ria under biofilm conditions.pH curves were created by measuring the pH values at different time points within 24 h.The anthrone sulfuric acid method was used to determine the effect of Xinjiang Mori Cortex extract on the synthesis levels of soluble and insoluble extracellular polysaccharides.Using lactate dehy-drogenase(LDH)activity assay and calcium ion(Ca2+)leakage assay,the effects of Xinjiang Mori Cortex extract on LDH content and Ca2+concentration in the supernatant of 3 cariogenic bacterial biofilms were determined.Using live and dead bacteria staining experiments,the effect of Xinjiang Mori Cortex extract on the biofilm activity of 3 cariogenic bacteria was determined.Results Compared with negative control group,the experimental group(excluding 1/4MBIC50 experimental group)and CHX group had biofilms,ΔpH values were decreased,and the ability of 3cariogenic bacteria to produce IEPS and SEPS in biofilms were decreased.The LDH content and Ca2+concentration in the supernatant was increased,and the total number of biofilm bacteria was decreased.The number of dead bacteria wasincreased,and the percentage of live/dead bacteria was decreased,with statistical significance(P＜0.05).Compared withCHX group,the experimental group had3 types of cariogenic bacterial biofilms,ΔpH values were increased,and the a-bility of biofilms to produce IEPS and SEPS were increased.The LDH content and Ca2+concentration in the supernatant wasdecreased,with statistical significance(P＜0.05).Conclusion Xinjiang Mori Cortex ex-tract has a certain inhibitory effect on the acid and sugar production ability of 3 cariogenic bacteria biofilms,which can affect the activity of destroying the biofilms of the 3 cariogenic bacteria.
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