Experimental study on tanti-caries effect of Xinjiang Mori Cortex extract
Objective To explore the effectiveness and safety of Xinjiang Mori Cortex extract on caries pre-vention through animal experiments.Methods An animal model of rat caries was established on the basis of previous in vitro studies,42 SPF-SD rats were stochastic divided into 6 groups,respectively Xinjiang Mori Cortex extract high,medium and low dose(1.00 g/L,0.50 g/L,0.25 g/L),positive control group(0.12％chlorhexidine,CHX group),negative control group(sterile distilled water intervention caries model group,CA group),blank control group(no intervention,Control group),by group continuous in-tervention rats for 3 weeks.(1)Drug effectiveness evaluation:rat saliva was collected,and streptococcus mutans was counted to determine the level of streptococcus mutans.To assess the progression of dental caries,rat jaw tissue was collected and the left upper mandible was stained with 0.4％ammonium vio-lonate for 18 hours,the socket and groove and smooth surface caries of the rat molars were scored under the microscope.The right upper mandible was made into the dental molar piece,stained with 0.1 mmol/L rhodamine B fluorescent dye for 24 hours,and the degree of demineralization of mouse molar teeth was ob-served by laser confocal microscope.(2)Drug safety evaluation:Recorded the weight increase of therats during the experiment,and observed the effect of Xinjiang Mori Cortex extract on the growth and develop-ment of therats.Calculated the coefficient of viscera(Organ weight/rat body weight 100％)to evaluate the effect of Xinjiang Mori Cortex extract on the main organs in rats.HE staining of the main viscera and buccal mucosa tissues wereperformed to observe the changes in tissue structure to evaluate the irritation of Xinjiang Mori Cortex extract on the oral mucosa and organ cytotoxicity.Results After 50 days of mouse age,compared with CA group,the high and medium dose groups of Xinjiang Mori Cortex extract showed a decrease in S.mutans levels,and the difference was statistically significant(P＜0.05).Compared with CA group,the high and medium dose groups of Xinjiang Mori Cortex extract showed a decrease in the key scores of smooth surface and caries lesions at all levels in the molars and grooves,while the low-dose group of Xinjiang Mori Cortex extract showed a decrease in smooth surface,E-grade,Ds grade and Dm grade scores.The high-dose group and CHX group showed a decrease in fluorescence penetration into en-amel or dentin,while the medium dose and CHX groups of Xinjiang Mori Cortex extract showed an in-crease in cardiac organ coefficients,with statistical significance(P＜0.05).During the experiment,the health status of each group of therats were good,with no dead individuals,and the growth trend of the rat weight was similar.The HE staining sections of the main organs and cheek mucosa of each group of ther-ats were uniform,and there were no significant changes in the histological structure.Conclusion The ex-tract of Xinjiang Mori Cortex extract can effectively prevent the occurrence and progression of dental caries in rats,and ithas no significant impact on the weight and function of major organs of the rats.It is also non irritating to the oral mucosa.
Xinjiang Mori Cortexanti-cariesanimal modeleffectivenesssafety
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