Preliminary observation and analysis of effect of ornithine decarboxylase 1(ODC1)gene on PC-3 LncRNA expression in prostate cancer cells
Objective To explore the effect of ornithine decarboxylase 1(ODC1)gene on PC-3 LncRNA ex-pression in prostate cancer cells,and to provide some experimental basis and clues for further study of its role and regulation mechanism.Methods Prostate cancer cell PC-3 was selected as the research object,ODC1 gene was knocked down,q-PCR detection and WB detection were verified and Illumina Novaseq 6000 and PE150 models were used for high-throughput sequencing.Coding potential analysis method(CPC analysis,CNCI analysis,CPAT analysis and LGC analysis)were used to predict and screen the se-quencing results,and the selected lncRNAs were analyzed by difference analysis,cis-acting element analy-sis and GO and KEGG enrichment analysis.Several functional lncRNAs reported to be closely related to prostate cancer were selected and verified by q-PCR.Results(1)341 lncRNA were selected in ODC1 knockdown PC-3 cells,with 154 upregulated and 128 downregulated in the differentially expressed ln-cRNA.There were 38 cis-regulated targets of eligible differential lncRNA being differentially expressed with differentially expressed genes.(2)GO and KEGG enrichment analysis of differential expressed lncR-NAs showed that lncRNA target genes were involved in various biological pathways in vivo,such as phos-phatidylinositol 3 kinase/protein kinase B signaling pathway(PI3K-Akt signaling pathway),Ras signaling pathway,and were also closely related to tumor angiogenesis,cell population proliferation,and positive regulation of cell division.(3)q-PCR validation results:LINC00973 and TERC was significantly downreg-ulated,and LINC00638 was significantly upregulated,which was consistent with the differential expres-sion of lncRNA obtained by GO and KEGG enrichment analysis.Conclusion Knockdown of ODC1 leads to differential expression of lncRNAs in PC-3 cells,and it is possible that ODC1 gene affects cell signaling pathway through lncRNAs to promote the occurrence and development of tumors.This experiment pro-vides some new experimental basis for further investigating the role of the ODC1 gene in prostate cancer.
万方数据
维普
