Expression of postsynaptic density protein-95 in exocrine bodies of triple negative breast cancer and its correlation with clinicopathological features
Objective To investigate the expression of messenger RNA(mRNA)postsynaptic density pro-tein 95(PSD-95)in serum exocrine of the patients with triple negative breast cancer and its relationship with pathological features.Methods Theserum exocrine bodies were collected from 4 patients with triple negative breast cancer and 4 patients with benign breast tumor(fibroadenoma and tumor-like hyperplasi-a).The mRNAs with significant difference between triple negative breast cancer and benign breast tumor was screened by high-throughput microarray and gene chip.The functional enrichment of each mRNA was understood by enrichment analysis,and it was found that the differentially expressed mRNA(PSD-95,al-so known as DLG4)was enriched in the NF-κB signal pathway,which was related to tumor invasion and metastasis.The histopathological sections of 30 cases of benign breast tumor and 36 cases of triple nega-tive breast cancer were analyzed by immunohistochemical staining,and the expression level of PSD-95 in triple negative breast cancer and its correlation with clinicopathological features were verified.Results The ex-pression level of PSD-95 in the tissues of the patients with triple negative breast cancer was significantly lower than that in patients with benign breast tumors.The results of correlation analysis showed that the differential expression of PSD-95 was significantly correlated with lymph node metastasis(P＜0.05).There was no correlation with age,menopausal status,Ki67 expression level,tumor size(T)and histo-logical grade.Conclusion The expression of DLG4 in triple negative breast cancer is significantly down-regulated,which is negatively correlated with lymph node metastasis,and it may be related to the invasion and metastasis of breast cancer.
triple negative breast cancerexocrinepostsynaptic density protein 95(PSD-95,DLG4)inva-sion and metastasis
万方数据
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