Effect of Tangshen enema formula combined with valsartan in treatment of type 2 diabetic nephropathy and its effect on expression of toll like receptor 2(TLR2)and toll like receptor 2(TLR4)in kidney tissue of rats
Objective To observe the effect of Tangshen enema formula combined with valsartan on type 2 diabetic nephropathy and the expression of toll like receptor 2(TLR2)and C(TLR4)in kidney tissue of rats.Methods 60 8-week-old female SD rats without specific pathogen free(SPF)were selected.After adaptive feeding for 7 days,10 rats were randomly selected as blank control group,and the other 50 rats underwent right kidney extraction surgery to construct the rat model of type 2 diabetic nephropathy.10 rats died during the modeling process.40 rats with successful modeling were randomly divided into model group,Tangshen enema group(Tangshen enema 0.13 ml/10 g),valsartan intervention group(valsartan 0.12 mg/10g),valsartan+Tangshen enema group(Tangshen enema 0.13 ml/10g combined with valsar-tan 0.12 mg/10 g),once a day for 4 weeks.The blood glucose level,renal histomorphological changes,urinary protein,urea nitrogen(BUN)and creatinine(CR)levels,renal wet weight and renal hypertrophy index,TLR2,TLR4 messenger RNA(mRNA)and protein expression were observed in the 5 groups.Re-sults Compared with blank control group,the levels of blood glucose and urinary protein in each model group were significantly increased(P＜0.05);Compared with model group,the blood glucose of Tangsh-en enema,valsartan intervention group and valsartan+Tangshen enema formula intervention group was decreased,but the difference was not statistically significant(P＞0.05).The urinary protein content of Tangshen enema formula intervention group,valsartan tablets intervention group and valsartan+Tangsh-en enema formula intervention group was decreased(P＜0.05),and the urinary protein content of valsar-tan+Tangshen enema formula intervention group was decreased significantly(P＜0.05).Compared with blank control group,the renal tissue lesions in the model group were significant,such as renal tubular ex-pansion,fibrosis,inflammatory cell infiltration and necrotic cell shedding;Compared with model group,the degree of renal tubule expansion,inflammatory cell infiltration and necrotic cell shedding in the Tang-shen enema intervention group and valsartan tablets intervention group were reduced,while the renal tu-bules in the Valsartan+Tangshen enema intervention group had no expansion,only a small amount of in-flammatory cell infiltration and necrotic cell shedding,and the renal structure was closer to the blank con-trol group.Compared with blank control group,the serum Cr content,fecal Cr content,serum BUN con-tent,blood/fecal bun ratio,renal wet weight,renal hypertrophy index,TLR2mRNA,TLR4mRNA,TLR2 protein and TLR4 protein expression in the model group were significantly was increased(P＜0.05);Compared with model group,the above indexes in Tangshen enema formula intervention group,valsartan intervention group and valsartan+Tangshen enema formula intervention group was decreased(P＜0.05),and the decrease in valsartan+Tangshen enema formula intervention group was the largest.Conclusion Tangshen enema formula combined with valsartan has better effect in the treatment of type 2 diabetic nephropathy,and it can inhibit the expression of TLR2 and TLR4 in rat kidney tissue,so it is worthy of clinical application.
万方数据
维普
