Objective To investigate the early effects of Echinococcus granulosus infection on natural killer T(NKT)cells and its related factors in mouse liver.Methods 24 female C57BL/6 mice were randomly assigned to control group and infection group,with 12 mice in each.The infection group received inocula-tion of Echinococcus granulosus protoscoleces via the hepatic portal vein,while the control group received an equivalent volume of saline.After 4 weeks,liver tissues were harvested from 6 mice per group for Mas-son staining,and the tissues from the remaining mice were used to isolate lymphocytes for flow cytometry analysis of various NKT cell subsets and their cytokine expression,including interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin-4(IL-4),interleukin-10(IL-10)and interleukin-17A(IL-17A).Results Compared with control group,the invasion sites and adjacent liver tissues in the infection group showed connective tissue disease,obvious fibrosis,a significant increase in the proportion of fibers and a wide distribution of fibers,the granuloma was surrounded by a cord-like fibrous septum.Compared with control group,the ratio of NKT cells and CD4+NKT cells in the liver of the infected group were decreased significantly(P＜0.01),the ratio of TNF-α secreted by NKT cells and the ratio of TNF-α was secreted by CD4+NKTcells in the liver of infected mice increased significantly(P＜0.01).Conclusion In the early stage of Echinococcus granulosus infection,the degree of liver fibrosis and the level of TNF-α produced by NKT cells and their subgroups CD4+NKT cells was increased,suggesting that liver NKT cells may par-ticipate in the early liver immune response to Echinococcus granulosus infection.
关键词
囊型包虫病/细粒棘球蚴/肝脏/自然杀伤T细胞
Key words
cystis echinoccosis/Echinococcus granulosus/liver/natural killer T cells
维普
万方数据