Effects of Echinococcus granulosus infection on liver natural killer T(NKT)cells and related factors in mice in early stage
Objective To investigate the early effects of Echinococcus granulosus infection on natural killer T(NKT)cells and its related factors in mouse liver.Methods 24 female C57BL/6 mice were randomly assigned to control group and infection group,with 12 mice in each.The infection group received inocula-tion of Echinococcus granulosus protoscoleces via the hepatic portal vein,while the control group received an equivalent volume of saline.After 4 weeks,liver tissues were harvested from 6 mice per group for Mas-son staining,and the tissues from the remaining mice were used to isolate lymphocytes for flow cytometry analysis of various NKT cell subsets and their cytokine expression,including interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),interleukin-4(IL-4),interleukin-10(IL-10)and interleukin-17A(IL-17A).Results Compared with control group,the invasion sites and adjacent liver tissues in the infection group showed connective tissue disease,obvious fibrosis,a significant increase in the proportion of fibers and a wide distribution of fibers,the granuloma was surrounded by a cord-like fibrous septum.Compared with control group,the ratio of NKT cells and CD4+NKT cells in the liver of the infected group were decreased significantly(P＜0.01),the ratio of TNF-α secreted by NKT cells and the ratio of TNF-α was secreted by CD4+NKTcells in the liver of infected mice increased significantly(P＜0.01).Conclusion In the early stage of Echinococcus granulosus infection,the degree of liver fibrosis and the level of TNF-α produced by NKT cells and their subgroups CD4+NKT cells was increased,suggesting that liver NKT cells may par-ticipate in the early liver immune response to Echinococcus granulosus infection.
cystis echinoccosisEchinococcus granulosuslivernatural killer T cells
万方数据
维普
