Study on effect of different functional compounds on formation of migrasomes
Objective To investigate the effects of 4 different functional compounds,SF1670,colchicine,metformin and aspirin on the formation of migrasomes.Methods The L929 Tspan4-mCherry cell line was constructed by Vigofect chemical transfection method.The cells were divided into control group(complete medium without drug),SF1670 group(4 μmol/L),colchicine group(0.6 μmol/L),metformin group(32μmol/L)and aspirin group(1 μmol/L),and treated for 11 hours,respectively.Live cell imaging experi-ment was used to photograph the migrasomes produced by the cells.Fiji-Image J was used to calculate the number of migrasomes produced and the formation area,diameter and length of retraction fibers.Results Con-focal imaging results showed that the L929 overexpressing Tspan4-mCherry cell line was successfully es-tablished,retraction fibers and migrasomes.Compared to the effect of 4 drugs on the migrasomes,the SF1670 group was decreased(P＜0.01),and the formation area and length of shrinking fibers were sig-nificantly inhibited(P＜0.000 1);the migrasomes number was decreased in colchicine group(P＜0.000 1),and the formation area of shrinking fibers was decreased(P＜0.05);and the formation area(P＜0.05)and diameter(P＜0.000 1)was decreased in metformin group.The aspirin was increased(P＜0.01)and the diameter of retraction fibers was slightly shortened(P＜0.05).Conclusion SF1670,col-chicine,metformin and aspirin have different effects on the formation of migrasomes and retraction fibers.Migrasomes may play an important role in tumors,type 2 diabetes,inflammation,myocardial infarction and other diseases.
NETL
NSTL
万方数据
