Expression and clinical prognosis of EMC6,Apaf-1 and Caspase-3 in cervical cancer tissues
Objective To investigate the expression of EMC6,Apaf-1 and Caspase-3 proteins in cervical cancer and to analyze their correlation with the clinical-pathological characteristics and prognosis of the pa-tients.Methods Immunohistochemical method was used to detect the expression of EMC6,Apaf-1 and Caspase-3 proteins in cervical cancer and adjacent non-cancerous tissues.Clinical-pathological data of the patients were collected for analysis.Spearman was used to detect the correlation between variables.Uni-variate and multivariate Cox regression analyses were conducted to identify prognostic factors.Results The positive expression rates of EMC6,Apaf-1 and Caspase-3 proteins in cervical cancer tissues were signifi-cantly lower than those in adjacent tissues(P＜0.05).There was no significant difference in the positive expression of EMC6 protein among different clinicopathological features(P＞0.05).The positive expres-sion of Apaf-1 protein in patients with different differentiation degrees of cervical cancer was significantly different(x2=4.723,P=0.030).The positive expression of Caspase-3 protein in cervical cancer patients with different clinical stages and differentiation degrees was significantly different(x2=4.495,P=0.034;x2=5.010,P=0.025).The correlation coefficients between EMC6 and Apaf-1,as well as between EMC6 and Caspase-3,were 0.626(P＜0.001)and 0.554(P＜0.001),respectively,indicating a positive correla-tion.Univariate test showed that pathological type(P=0.047),tumor size(P＜0.001),EMC6(P=0.004),Apaf-1(P=0.029)and Caspase-3(P=0.039)were prognostic factors affecting the overall survival of patients with cervical cancer.Multivariate analysis showed that EMC6(P=0.005)and tumor size(P＜0.001)were in-dependent influencing factors of poor prognosis in patients with cervical cancer.Conclusion In cervical cancer tissues,low expression of EMC6,Apaf-1 and Caspase-3 is associated with poor patient prognosis.EMC6 is positively correlated with Apaf-1 and Caspase-3,and both EMC6 and tumor size are independent prognostic factors.These proteins play significant roles in the progression of cervical cancer and have the potential to serve as prognostic markers and therapeutic targets.
NETL
NSTL
万方数据
