Dexmedetomidine improved postoperative cognitive dysfunction(POCD)in elderly rats by up-regulating miR-148a-3p and inhibiting apoptosis
Objective To explore the protective effect of dexmedetomidine on postoperative cognitive dys-function(POCD)in elderly rats and its specific mechanism.Methods 98 male SD rats aged 18 months were selected,with 18 as the normal control group,and the remaining 80 using sevoflurane and liver re-section surgery to establish a POCD model.After the model was established,the rats were randomly di-vided into model group,mode+saline group,model+dexmedetomidine group and model+dexmedeto-midine+atimezole group,with 20 rats in each group to explore the effect of dexmedetomidine on POCD in elderly rats.Then,four rats were selected from each of the model group and model+dexmedetomidine group,and given miR-148a-3p mimics or inhibitors to further study the mechanism of dexmedetomidine in improving POCD.Results Sevoflurane anesthesia+liver lobectomy leaded to a significant decline in post-operative cognitive function,an increase in neuroinflammation and pyroptosis,and a decrease in hippocam-pal synaptic plasticity and miR-148a-3p expression in rats(P＜0.05).Dexmedetomidine can improve postoperative cognitive function and the above pathological changes(P＜0.05).The effect of dexmedeto-midine on cognitive function was significantly reduced in the model+dexmedetomidine+atimezole group(P＜0.05).miR-148a-3p mimic could mimic the protective effect of dexmedetomidine on POCD and in-hibit pyroptosis,while miR-148a-3p inhibitor could reduce the protective effect of dexmedetomidine and promote the occurrence of pyroptosis(P＜0.05).FMR1 may be the downstream target of miR-148a-3p.Conclusion Dexmedetomidine can improve cognitive dysfunction,enhance the plasticity of hippocampus in anesthetized rats during surgery,alleviate neuroinflammation,and may inhibit the occurrence of pyropto-sis by up-regulating the expression of miR-148a-3p.FMR1 may be the downstream target of miR-148a-3p.
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