Screening of optimal labeling conditions of superparamagnetic iron oxide before MR tracing of rabbit adipose stem cells
Objective To label rabbit adipose mesenchymal stem cells(Adipose derived stem cells,ADSCs)with polylysine modified superparamagnetic iron oxide(Polylysine coated superparamagnetic iron oxide,PLL-SPIO)nanoparticles,to explore the effects of different SPIO concentration and incubation time on ADSCs labeling rate,survival rate and cell proliferation rate,and to select the optimal labeling conditions for SPIO labeling ADSCs.Methods The 3rd generation rabbit ADSCs was cultured with different concen-trations of SPIO(12,25,50 μg/mL)and incubated for different time(6,12,24,48 h).After the incu-bation,prussian blue staining was used to calculate the cell labeling rate,cytotoxicity test was used to de-tect the cell survival rate,and 6 groups of cells with higher cell survival rate were selected according to the results of cytotoxicity test.Results Prussian blue staining showed that the cell labeling rate and the num-ber of blue staining granules in each group was increased with the increase of SPIO concentration and the prolongation of incubation time.In the cytotoxicity test,the cell survival rate was not affected at lower SPIO concentration and shorter incubation time,and there was no significant difference among the 3 groups.With the increase of SPIO concentration and the prolongation of incubation time,the cell survival rate was decreased,and the decrease was the most obvious at 48 h.The results of cell proliferation experi-ments showed that the cell proliferation rates of the experimental groups decreased with the increase of SPIO concentration and the extension of incubation time;and after the cells entered the rapid growth peri-od,there were significant differences in the cell proliferation rates of the experimental groups compared with those of the negative control group,among which the differences in cell proliferation rates of the 12 μg/mL SPIO-6 h group and the 25 μg/mL SPIO-6 h group and the negative control group were smaller than those of the other groups.Conclusion According to the comprehensive evaluation of intracellular SPIO granule content,cell labeling rate,survival rate and proliferation rate,incubation with 25 μg/mL SPIO for 6 h is the best labeling condition for PLL-SPIO labeling ADSCs in this study.
superparamagnetic iron oxideadipose mesenchymal stem cells(ADSCs)incubation concen-trationincubation time
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