Study on the separation and purification of Caffeine from tea extract by virtual template MIPs SPE combined technology
Objective Catechins were usedas virtual templates to preparemolecular imprinting polymers(MIPs)and solid-phase extraction(SPE)technology to separate and purify caffeine from crude tea ex-tract.Methods A virtual template molecularly imprinted polymer was synthesized by in-situ polymeriza-tion using catechins as virtual template molecules,4-vinylpyridine as functional monomer,ethylene glycol dimethacrylate as crosslinking agent and polystyrene as macromolecular crowding reagent.On the basis of characterizing the imprinted polymer,it was used as solid phase extraction adsorbent to separate and purify caffeine from crude tea extracts.Results The optimal imprinting factor for caffeine in the prepared cate-chin molecularly imprinted polymer was 4.57.After separating and purifying caffeine from crude tea ex-tracts,the recovery rate of caffeine can reach over 85％under the condition of ensuring caffeine purity above 95％.Conclusion The molecularly imprinted polymer synthesized using catechin as a virtual tem-plate can be applied for the separation and purification of caffeine from tea extracts.
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