Experimental research of Rou Ji Shun Cui Yin on skeletal muscle insulin resistance in type 2 diabetes mellitus
Objective To investigate the effect of Rou Ji Shun Cui Yin on insulin resistance in skeletal mus-cle of T2DM and its mechanism.Methods A total of 40 db/db mice were randomly divided into model group,low-dose group,high-dose group and positive control group,with 10 mice in each group.The low-dose group was given 157.5 mg·g-1·d-1 gavage,the high-dose group was given 630 mg·g-1·d-1,and the positive control group was given 200 mg·g-1·d-1 metformin.There were 10 db/dm mice in the blank control group,and the mice were given the same dose of normal saline gavage.Themice in each group wass dosed 1 time per day for 12 weeks.At 6 weeks and 12 weeks after administration,the body weight,food intake and water intake of the mice were monitored.The levels of FBG,FINS,TC and TG in peripheral blood were measured.The degree of insulin resistance was assessed by the insulin resistance index(HOMA-IR).ELISA was used to determine the inflammatory cytokines IL-6 and TNF-α and glu-cose transporters GLUT1 and GLUT4 in peripheral blood.After 12 weeks,oral glucose tolerance test(OGTT)and insulin tolerance test(ITT)were used to evaluate the glucose metabolism of mice.The wet weight of skeletal muscle,the percentage of GLUT1 and GLUT4 positive expression and the pathological changes of skeletal muscle were compared among the 4 groups.Results Compared with the blank control group,the body weight,food intake,water intake,fasting blood glucose,fasting insulin level,OGTT blood glucose,IL-6,TNF-α,GLUT1,the ratio of GLUT1 positive expression in skeletal muscle and HO-MA-IR,the area under the curve were significantly increased in the model group(P＜0.05).However,the GLUT4 and the ratio of GLUT4 positive expression and the total wet weight of skeletal muscle in mice were significantly decreased(P＜0.05).Compared with the model group,the food intake,water intake,fasting blood glucose and fasting insulin,IL-6,TNF-α,GLUT1,area under the curve,GLUT1positive expression and HOMA-IR ratio in low-dose,high-dose groups and positive control groups were significant-ly decreased(P＜0.05).However,GLUT4 and total wet weight of skeletal muscle of mice were signifi-cantly increased(P＜0.05),and the increase of GLUT4,GLUT4 postiive expression and total wet weight of skeletal muscle of mice in the high-dose group was more significant than that in the low-dose group(P＜0.05).The ratio of HOMA-IR and GLUT1 in skeletal muscle tissue wasdecreased significantly in the high-dose group than in the low-dose group(P＜0.05).Compared with the positive control group,the to-tal wet weight of skeletal muscle of the mice in the low and high dose groups was increased(P＜0.05).Compared with blank control group,the skeletal muscle tissue in the model group showed irregular polyg-onal depression,sparse muscle fibers,disordered arrangement and myolysis in some myocytes.In the low-dose group and the high-dose group,the muscle fibers of skeletal muscle tissue increased to varying de-grees,showing regular polygonal masses,and the abnormal morphology of muscle cells was reduced,and the arrangement was neat.There was no obvious myolysis.Conclusion By improving skeletal muscle IR,lowering blood sugar and improving T2DM,the mechanism of Rou Ji Shun Cui Yin is related to increasing the wet weight of skeletal muscle,improving the pathological morphology of skeletal muscle,reducing GLUT1 and increasing GLUT4 in skeletal muscle tissue.
Rou Ji Shun Cui Yinskeletal muscleinsulin resistance
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