Application of a rechargeable animal telemetry system for ECG monitoring in natural state mice
Objective To explore the application of a rechargeable animal telemetry system for ECG moni-toring in naturally awake mice and to provide ECG parameters in naturally awake mice.Methods 20 C57 mice(half male and half female)were fitted with implantable electrodes,and cardiac activity was continu-ously recorded and analyzed for cardiac parameters throughout the day for 24 hours.Results 20 C57 mice were monitored for 24 hours.The average heart rate of C57 mice was(493.55±73.29)beats per minute throughout the day.The average heart rate of female mice was(522.83±63.70)beats per minute throughout the day,with a maximum heart rate of 673.60 beats per minute and a minimum heart rate of 385.90 beats per minute.The average heart rate of male mice was(464.27±70.55)beats per minute throughout the day,with a maximum heart rate of 641.60 beats per minute and a minimum heart rate of 321.50 beats per minute.The average heart rate of female mice was faster than that of male mice,and the difference was statistically significant(P＜0.05).The average heart rate of male mice was faster at night than during the day(P＜0.05).The heart rate change indicators in the daytime(07:00-19:00)and nighttime(19:00-the next day 07:00)of male and female mice showed that the average heart rate of fe-male mice increased faster during the day than at night.The average heart rate of male mice during the day was slower than that at night,and the difference was statistically significant(all P＜0.05).The electro-cardiographic indicators in anesthetized mice showed that there were statistically significant differences in P wave amplitude,P wave duration,and RR interval between male and female mice(P＜0.05);the QRS duration of male mice was longer than that of female mice,and the difference was statistically significant(P＜0.05);there were no statistically significant differences in QRS amplitude and QT interval between male and female mice(P＞0.05).Conclusion The rechargeable animal telemetry system can safely and minimally impair 24-hour ECG parameter recordings in freely moving mice.The recorded signals are clear and stable,and can be used to monitor heart rate and rhythm in the natural state of normal and acute and chronic model mice.
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