To explore mechanism of Qingdu Tiaogan decoction in treating hepatic fibrosis based on sinusoid capillarization
Objective To explore the mechanism of Qingdu Tiaogan decoction in treating liver fibrosis by improving hepatic sinusoid capillarization.Methods 60 male SD rats were selected,with 10 as the blank group and the remaining 50 rats established a liver fibrosis model by tail vein injection of ConA.After suc-cessful modeling,the model rats were randomly divided into a model control group,a sorafenib group,and low,medium,and high dose groups of Qingdu Tiaogan decoction,with 10 rats in each group.The blank group and model control group were not intervened,while the other groups of rats were orally ad-ministered sorafenib or different doses of Qingdu Tiaogan decoction once a day for 5 consecutive weeks.By observing the morphological changes of rat liver tissue under light microscopy,scanning electron microsco-py was used to observe the changes in the hepatic sinusoidal endothelial cell window,and enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and immunoglobulin G(IgG).Immunohistochemistry(IHC)was used to detect the expression of laminin(LN)and type Ⅳ collagen(Col Ⅳ)in liver tissue.Immunoblot-ting(WB)technology was used to detect the expression of platelet endothelial cell adhesion molecule(CD31)and von Willebrand factor(vWF)in liver tissue,and real-time fluorescence quantitative polymer-ase chain reaction(qPCR)was used to detect the expression of vascular endothelial growth factor(VEGF)mRNA.Results Compared with the blank group,the model control group showed severe liver tissue damage in rats,manifested as hepatocyte necrosis,vacuolar formation,fibrous tissue proliferation,re-duced hepatic sinusoidal endothelial cell fenestration,and increased basement membrane.Serum ALT,AST,IgG levels,and LN,Col Ⅳ,CD31,vWF,VEGF mRNA expression were significantly increased(P＜0.05).Compared with the model control group,the low,medium,and high dose groups of Qingdu Tiaogan decoction can alleviate liver tissue damage,intervene in the de fenestration of liver sinusoidal en-dothelial cells,significantly reduce ALT,AST,IgG levels,and LN,Col Ⅳ,CD31,vWF,VEGF mRNA expression(P＜0.05).Conclusion Qingdu Tiaogan decoction can alleviate liver injury,improve liver function,enhance immune levels,intervene in the de fenestration of hepatic sinusoidal endothelial cells and deposition of subendothelial basement membrane,downregulate the expression of LN,Col Ⅳ,CD31,vWF,and VEGF mRNA,thereby inhibiting angiogenesis,improving hepatic sinusoidal capillarization,and reducing the occurrence of liver fibrosis.
Qingdu Tiaogan decoctionhepatic fibrosissinusoid capillarizationangiogenesismechanism of action
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维普
