Effects of acupoint catgut embedding on cognitive function and levels of 5-HT,DA and GABA in insomnia rats
Objective To observe effects of catgut implantation at acupoint treatment on expression of 5-hydroxytryptamine(5-HT),dopamine(DA)and γ-aminobutyric acid(GABA)in hypothalamus of PCPA insomnia model rats,and to investigate possibilities of mechanisms of catgut implantation in treatment of insomnia.Methods Twenty-four SPF SD male rats were selected,6 of them served as blank group,and the other 18 rats were made insomnia model by intraperitoneal injection of PCPA suspension.After suc-cessful modeling,the model rats were randomly divided into model control group,acupuncture group and acupoint catgut embedding group,6 rats each.The rats in control group and model group only grasped without intervention;acupuncture group needled Baihui,Shenmen(bilaterally)and Sanyinjiao(bilaterally),1 d/times for 14 days;burying thread group buried threads,with the same points as acupuncture group,for 7 d/times twice in total.Sodium pentobarbital synergistic sleep experiment observed sleep latency and sleep duration in rats;the Morris water maze experiment investigated the rats'capacity of spatial cogni-tion;HE staining analyzed the neuronal cell of hypothalamus tissues;5-HT,DA,and GABA protein con-tents of hypothalamus tissues tested with ELISA method.Results Compared with model group,the sleep latency of rats in each treatment group was shortened and the sleep duration was prolonged(P＜0.05).Compared with model group,the escape latency of rats in each treatment group were decreased and the number of times of crossing the hidden platform wereincreased(P＜0.05).Compared with model group,the nerve cells in the hypothalamus area were arranged in a messy and loose manner,the gap was in-creased,and the neuroinflammation such as empty package was improved after the treatment.Compared with model group,the concentration of GABA and 5-HT protein in each treatment group was increased,the concentration of DA protein was decreased(P＜0.05),and the concentration of DA protein was de-creased significantly(P＜0.05).Conclusion Catgut embedding therapy can effectively improve insomnia,anxie-ty symptoms and cognitive function such as learning and memory in insomnia rats.The mechanism may be related to the regulation of neurotransmitters GABA,5-HT and DA concentrations in the hypothalamus.
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