Expression and significance of killer cell lectin-like receptor G1(KLRG1)and T cell immunoglobulin and ITIM domains(TIGIT)
Objective To investigate the difference of killer cell lectin-like receptor G1(KLRG1)and T cell immunoglobulin and ITIM domains(TIGIT)expression levels on CD4+T,CD8+T,NK and NKT cells in peripheral blood,cancer and adjacent tissue lymphocytes of the patients with gastric cancer,and to explore the relationship between KLRG1 and TIGIT expression levels and clinical features,prognosis and immune efficacy.Methods Primary gastric cancer patients who were feasible for surgery in the hospital from Janu-ary 2022 to December 2023 were included,36 cases of cancerous tissues and 31 cases of paracancerous tis-sues were retained,and peripheral blood was collected from 15 cases before the treatment.The expres-sions of KLRG1 and TIGIT on CD4+T,CD8+T,NK and NKT cells in PBMCs,tumor and adjacent TILs were detected by flow cytometry.The relationship between its expression and clinical features was ana-lyzed.The prognostic value of KLRG1 and TIGTI in gastric cancer used by Kaplan-Meier plotter online database.Immunophenotypic scores were obtained from the TCIA database to study the sensitivity of KLRG1 and TIGIT expression to immunotherapy.Results(1)The expression of KLRG1+CD8+T cells,KLRG1+NKT cells,TIGIT+CD8+T cells,TIGIT+CD4+T cells,TIGIT+NKT,TIGIT+NK,KLRG1+TIGIT+CD8+T cells and KLRG1+TIGIT+CD4+T cells in tumor were significantly higher than that of ad-jacent tissues(P＜0.05).(2)The expression of KLRG1+CD8+T cells and KLRG1+NKT cells in periph-eral blood was significantly higher than that in cancer tissues(P＜0.05).However,the expression of TIGIT CD4+T and TIGIT+CD8+T cells in peripheral blood was lower than that in cancer tissues(P＜0.05).(3)KLRG1+CD4+T cells,KLRG1+CD8+T cells and KLRG1+NKT cells were positively correlated withCA199(r=0.37,0.45,0.40,P＜0.05)and KLRG1+NKT cells were also positively correlated with CA125(r=0.33,P＜0.05).(4)The median overall survival time,median first progression survival time and median post-progression survival time of the patients with high KLRG1 expression group were signifi-cantly shorter than those with low KLRG1 expression group(P＜0.05).(5)In predicting the efficacy of immunotherapy,the high KLRG1 and TIGIT expression group responded better than the low expression group to anti-PD-1 therapy and the combination of anti-PD-1 and anti-CTLA4 therapy(P＜0.001).Con-clusion KLRG1 and TIGIT are highly expressed in gastric cancer tissues.The high expression of KLRG1 in gastric cancer is related to the poor prognosis of gastric cancer.The expression of KLRG1 and TIGIT can effectively predict the immunotherapy response in gastric cancer patients.
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