Correlation study of CD94 and NKG2A expression and clinical features in gastric cancer
Objective To investigate the expression differences of CD94 and NKG2A on CD4+T and CD8+T cells in cancer tissues,adjacent tissues and peripheral blood of the patients with cancer and its correlation with clinical characteristics.Methods From January 2022 to December 2023,newly treated and operable gastric cancer patients were collected in the hospital.Finally,43 cases of cancer tissues,37 cases of adja-cent tissues and 14 cases of peripheral blood before the treatment were enrolled.The expression of CD94 and NKG2A on CD4+T and CD8+T cells in peripheral blood mononuclear cells,tumor infiltrating lym-phocytes in cancer tissues and adjacent tissues was detected by flow cytometry.The correlation between the expression and the clinical characteristics of gastric cancer was analyzed and compared.Results(1)The expression of CD94+CD8+T cells in cancer tissues was higher than that in peripheral blood and para-cancer tissues(both P＜0.05),the expression of NKG2A+CD4+T cell group in cancer tissue was higher than that in para-cancer tissue.The expressions of NKG2A+CD8+T and CD94+NKG2A+CD8+T cells in cancer tissues were higher than those in peripheral blood(both P＜0.001).(2)The expression of PD-1,CD103 and Tim3 in NKG2A+subgroup was higher than that in NKG2A-subgroup in CD8+T cells of cancer tissues of the patients(P＜0.001).(3)The high expression of CD94+NKG2A+CD8+T cells in cancer tissues and peripheral blood were significantly correlated with the status of p53(+)(P＜0.05).(4)Kaplan-Meier plotter analysis showed that the high expression of CD94,NKG2A and HLA-E sugges-ted that the OS and FP of GC patients were significantly prolonged(P＜0.05).Conclusion CD94 and NKG2A are prominently expressed in gastric cancer.NKG2A has the characteristic of tissue-resident mem-ory T cells.CD94+NKG2A+is correlated with p53 expression.CD94 and NKG2A may be predictive bio-markers and effective target for T cell immunotherapy in the development and progression of gastric cancer.
万方数据
维普
