Effect of echinacoside(ECH)on lowering uric acid and protecting liver and kidney in chronic hyperuricemia(HUA)mice
Objective To study the effect of echinacoside(ECH)in Cistanche tubuLosa(Schenk)on lower-ing uric acid and protecting liver and kidney in chronic hyperuricemia(HUA)mice.Methods 72 male ICR mice were randomly divided into a blank group,a model group,a positive drug group(non buxostat 8 mg/kg body weight),and ECH low,medium,and high dose groups(ECH 50,100,150 mg/kg body weight),with 12 mice in each group.Except for the blank group,all other groups of mice were orally ad-ministered hypoxanthine(500 mg/kg body weight)and potassium oxonate(1000 mg/kg body weight)dai-ly to establish a chronic HUA model for 42 days.Starting from the 14th day of modeling,except for the blank group and the model group,all other groups were orally administered with the corresponding drugs every day for a total of 28 days.Measured the activities of urea nitrogen(UREA),uric acid(BUA),cre-atinine(CREA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phospha-tase(ALP)in the serum of each group,as well as the activities of xanthine oxidase(XOD)and adenosine deaminase(ADA)in the serum.Observed the pathological changes of liver and kidney tissues in each group using hematoxylin eosin staining method.Results Compared with the blank group on the 42nd day of modeling,the water intake of the model group increased(P<0.01);Compared with the model group on the 42nd day of modeling,the water intake of the positive drug group and the low,medium,and high-dose ECH groups decreased,and the difference was statistically significant(P<0.01).Compared with blank group,the levels of serum CREA and UREA in the model group mice were decreased,while the level of serum BUA wasincreased,and the difference was statistically significant(P<0.01),indicating the suc-cessful establishment of the chronic HUA mouse model.Compared with model group,the high-dose ECH group showed a statistically significant decrease in serum UREA levels(P<0.05);The serum CREA lev-els wereincreased and BUA levels were decreased in the positive drug group and the low,medium,and high-dose ECH groups,with statistical significance(P<0.01).Compared with blank group,the serum AST and ALT levels,serum XOD and ADA activity was increased in the model group mice,and the differences were statistically significant(P<0.01).Compared with model group,the low,medium,and high dose groups of ECH showed a decrease in serum ALP levels in mice.The serum AST and ALT lev-els,as well as XOD and ADA activity,was decreased in the positive drug group and ECH medium and high-dose groups of the mice,with statistically significant differences(P<0.05;P<0.01).Pathological analysis results showed that ECH can improve liver and kidney damage caused by HUA.Conclusion ECH in Cistanche deserticola has the effect of reducing serum uric acid and protecting liver and kidney function in chronic HUA mice.