首页|松果菊苷对慢性高尿酸血症小鼠的降尿酸及肝肾保护作用研究

松果菊苷对慢性高尿酸血症小鼠的降尿酸及肝肾保护作用研究

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目的 研究管花肉苁蓉中的活性单体松果菊苷(Echinacoside,ECH)对慢性高尿酸血症(Hyperuricemia,HUA)小鼠的降尿酸效果及肝肾保护作用.方法 将72只雄性ICR小鼠随机分为:空白组、模型组、阳性药物组(非布司他8 mg/kg体重)、ECH低、中、高(ECH50、100、150 mg/kg体重)剂量组,每组12只.除空白组,其余各组小鼠每天灌胃次黄嘌呤(500 mg/kg体重)和氧嗪酸钾(1 000 mg/kg体重)建立HUA慢性模型,共42 d.造模第14天起,除空白组和模型组,其余各组每天灌胃相应的药物,共28 d.测定各组血清中尿素氮(Urea nitrogen,UREA)、尿酸(Uric acid,BUA)、肌酐(Creatinine,CREA)、谷氨酸氨基转移酶(Alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(Aspartate aminotransferase,AST)、碱性磷酸酶(Alkaline phospha-tase,ALP),以及血清中黄嘌呤氧化酶(Xanthine oxidase,XOD)和腺苷脱氨酶(Adenosine deami-nase,ADA)的活力.采用苏木精-伊红染色法观察各组肝肾组织的病理变化.结果 与空白组造模第42天比较,模型组饮水量增加(P<0.01);与模型组造模第42天比较,阳性药物组和ECH低、中、高剂量组饮水量降低,差异有统计意义(P<0.01).与空白组比较,模型组小鼠血清CREA和UREA水平降低,血清BUA水平升高,差异有统计意义(P<0.01),表明慢性HUA小鼠模型建立成功.与模型组比较,ECH高剂量组血清UREA水平降低,差异有统计意义(P<0.05);阳性药物组和ECH低、中、高剂量组血清CREA水平升高,BUA水平降低,差异有统计意义(P<0.01).与空白组比较,模型组小鼠血清AST和ALT水平升高,血清XOD和ADA活力增加,差异有统计意义(P<0.01).与模型组比较,ECH低、中、高剂量组小鼠血清ALP水平降低;阳性药物组和ECH中、高剂量组小鼠血清AST、ALT水平降低,XOD和ADA活力减小,差异有统计意义(P<0.05;P<0.01).病理学分析结果显示,ECH可改善HUA引起的肝脏和肾脏损伤.结论 管花肉苁蓉中的ECH对慢性HUA小鼠具有降低血清尿酸效果,保护肝肾功能的作用.
Effect of echinacoside(ECH)on lowering uric acid and protecting liver and kidney in chronic hyperuricemia(HUA)mice
Objective To study the effect of echinacoside(ECH)in Cistanche tubuLosa(Schenk)on lower-ing uric acid and protecting liver and kidney in chronic hyperuricemia(HUA)mice.Methods 72 male ICR mice were randomly divided into a blank group,a model group,a positive drug group(non buxostat 8 mg/kg body weight),and ECH low,medium,and high dose groups(ECH 50,100,150 mg/kg body weight),with 12 mice in each group.Except for the blank group,all other groups of mice were orally ad-ministered hypoxanthine(500 mg/kg body weight)and potassium oxonate(1000 mg/kg body weight)dai-ly to establish a chronic HUA model for 42 days.Starting from the 14th day of modeling,except for the blank group and the model group,all other groups were orally administered with the corresponding drugs every day for a total of 28 days.Measured the activities of urea nitrogen(UREA),uric acid(BUA),cre-atinine(CREA),alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phospha-tase(ALP)in the serum of each group,as well as the activities of xanthine oxidase(XOD)and adenosine deaminase(ADA)in the serum.Observed the pathological changes of liver and kidney tissues in each group using hematoxylin eosin staining method.Results Compared with the blank group on the 42nd day of modeling,the water intake of the model group increased(P<0.01);Compared with the model group on the 42nd day of modeling,the water intake of the positive drug group and the low,medium,and high-dose ECH groups decreased,and the difference was statistically significant(P<0.01).Compared with blank group,the levels of serum CREA and UREA in the model group mice were decreased,while the level of serum BUA wasincreased,and the difference was statistically significant(P<0.01),indicating the suc-cessful establishment of the chronic HUA mouse model.Compared with model group,the high-dose ECH group showed a statistically significant decrease in serum UREA levels(P<0.05);The serum CREA lev-els wereincreased and BUA levels were decreased in the positive drug group and the low,medium,and high-dose ECH groups,with statistical significance(P<0.01).Compared with blank group,the serum AST and ALT levels,serum XOD and ADA activity was increased in the model group mice,and the differences were statistically significant(P<0.01).Compared with model group,the low,medium,and high dose groups of ECH showed a decrease in serum ALP levels in mice.The serum AST and ALT lev-els,as well as XOD and ADA activity,was decreased in the positive drug group and ECH medium and high-dose groups of the mice,with statistically significant differences(P<0.05;P<0.01).Pathological analysis results showed that ECH can improve liver and kidney damage caused by HUA.Conclusion ECH in Cistanche deserticola has the effect of reducing serum uric acid and protecting liver and kidney function in chronic HUA mice.

xanthine oxidaseadenosine deaminasehyperuricemia(HUA)echinacoside(ECH)hepatore-nal protection

冯顺钱、阿热孜古丽·阿不拉、罗福详、马慧丽、李建梅、高莉、闫明

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新疆医科大学药学院,乌鲁木齐 830017

新疆维吾尔自治区维吾尔医药研究所,新疆维吾尔医方剂学重点实验室,乌鲁木齐 830049

黄嘌呤氧化酶 腺苷脱氨酶 高尿酸血症 松果菊苷 肝肾保护

2024

新疆医科大学学报
新疆医科大学

新疆医科大学学报

CSTPCD
影响因子:0.76
ISSN:1009-5551
年,卷(期):2024.47(12)