新疆医科大学学报2024,Vol.47Issue(12) :1644-1650.DOI:10.3969/j.issn.1009-5551.2024.12.015

准噶尔铁线莲质量标准的初步研究

Preliminary study on quality standard of Clematis songarica Bge.

胡月月 王芳 刘艺 赵媛
新疆医科大学学报2024,Vol.47Issue(12) :1644-1650.DOI:10.3969/j.issn.1009-5551.2024.12.015

准噶尔铁线莲质量标准的初步研究

Preliminary study on quality standard of Clematis songarica Bge.

胡月月 1王芳 2刘艺 1赵媛1
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作者信息

  • 1. 乌鲁木齐市中医医院药学部,乌鲁木齐 830000
  • 2. 乌鲁木齐市中医医院医务部,乌鲁木齐 830000
  • 折叠

摘要

目的 初步建立准噶尔铁线莲的质量标准.方法 通过显微分析观察准噶尔铁线莲粉末的微观结构;采用薄层色谱对准噶尔铁线莲中芦丁和齐墩果酸进行定性分析.采用高效液相色谱法测定10个不同产地准噶尔铁线莲中芦丁的含量,并建立准噶尔铁线莲的指纹图谱.结果 通过显微镜观察到准噶尔铁线莲粉末中的石细胞、晶鞘纤维、具缘纹孔导管等特征结构.通过薄层色谱实验确定准噶尔铁线莲中含有芦丁和齐墩果酸.10个不同产地准噶尔铁线莲中芦丁的含量在0.079~0.270 mg/g之间.建立的准噶尔铁线莲指纹图谱中,10个不同产地的准噶尔铁线莲图谱与对照图谱相似度均大于90%.结论 本研究为准噶尔铁线莲质量标准的建立提供了理论依据.

Abstract

Objective To set up the quality control of Clematis songarica Bge.Methods The microstruc-ture of J ungar iron lotus powder was observed by microscopic analysis.The qualitative analysis of rutin and oleanolic acid in Jungar iron lotus was carried out by thin-layer chromatography.High performance liquid chromatography was used to determine the content of rutin in 10 different regions of Jungar iron lo-tus,and the fingerprint spectrum of Jungar iron lotus was established.Results The characteristic struc-tures of stone cells,crystal sheath fibers and bordered pore ducts in the powder of Clematis songarica Bge.were observed under a microscope.Through thin-layer chromatography experiments,it was deter-mined that the Clematis songarica Bge.contained rutin and oleanolic acid.The content of rutin in 10 dif-ferent regions ofClematis songarica Bge.ranged from 0.079 to 0.270 mg/g.In the established fingerprint map of Clematis songarica Bge.,the similarity between the maps of 10 different origins of Clematis songarica Bge.and the control map was greater than 90%.Conclusion This study provides a theoretical basis for the establishment of quality standards for Clematis songarica Bge..

关键词

准噶尔铁线莲/质量标准/含量测定:初步研究

Key words

Clematis songarica Bge./quality control/content determination/preliminary study

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出版年

2024
新疆医科大学学报
新疆医科大学

新疆医科大学学报

CSTPCD
影响因子:0.76
ISSN:1009-5551
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