Study on the mechanism of emodin against osteoporosis based on network pharmacology and molecular docking technology
Objective To explore the mechanism of emodin in the treatment of osteoporosis by network pharmacological method and molecular docking technology.Methods The Pubchem database was used to obtain the chemical representative structure of emodin,the TCM systematic pharmacology database and analysis platform(TCMSP)database was used to retrieve the molecular target of emodin,the DisGenNet database was used to obtain the treatment related target of osteoporosis,and Venny 2.1.0 website was used to build a Venny map to screen the potential target of emodin and the common target of osteoporosis,Import the Cytoscape 3.2.1 software to build the"emodin target"network diagram,use R software and Bioconductor toolkit to conduct the function enrichment analysis of key target genes GO and KEGG,analyze the mechanism of emodin in treating osteoporosis through literature,and screen target proteins with low binding capacity.Results 31 emodin targets were retrieved from TCMSP database.In addition,1084 osteoporosis targets were obtained from DisGenNet,including 14 targets.After PPI network analysis,13 of them were included in the network.R software and Bioconductor toolkit were used to analyze GO and KEGG function enrichment of key target genes.Emodin may regulate IL-17 signal pathway,MAPK signal pathway Tumor necrosis factor signal pathway and PPAR signal pathway treat osteoporosis.Conclusion Emodin can play an antiosteoporosis role through multiple pathways and targets,and may play a specific biological role through HSP90AA1,SLC2A1,PPARG and MYC proteins.
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