非小细胞肺癌中PD-L1的表达与EGFR、ALK、KRAS基因突变的相关性及预后分析
The expression of PD-L1 in non-small cell lung cancer and its correlation with EGFR、ALK、KRAS gene mutation and its prognosis
袁轶群 1魏龙 2孙振柱3
作者信息
- 1. 生产建设兵团第一师医院病理科,新疆阿克苏 843000
- 2. 生产建设兵团第一师医院呼吸与危重症医学科一区,新疆阿克苏 843000
- 3. 新疆维吾尔自治区人民医院病理科,乌鲁木齐 830001
- 折叠
摘要
目的 探讨PD-L1在非小细胞肺癌(Non-small cell lung cancer,NSCLC)中的表达状态,分析PD-L1与EGFR、ALK、KRAS的相关性及其与NSCLC预后的关系.方法 收集2017年1月-2022年4月第一师医院病理科确诊的164例NSCLC患者,采用免疫组化法及运用二代测序技术分别检测PD-L1的表达和EGFR、ALK、KRAS基因状态,分析NSCLC患者中PD-L1的表达与EGFR、KRAS、ALK基因的相关性及预后.结果 PD-L1表达与年龄、性别、吸烟、种族、组织学、分期等均无关(P＞0.05);根据相关性分析,PD-L1表达与EGFR基因突变呈负相关(R=-0.251,P＜0.01),与ALK基因突变呈正相关性(R=0.232,P＜0.01),与BRAF基因突变不相关(R=-0.036,P＞0.05);通过1年期的生存性分析,PD-L1表达阳性的患者为67.9％,阴性的患者为84.1％.Kaplan-Meier生存分析:PD-L1阳性表达组的患者低于阴性表达组(x2=4.505,P＜0.05).结论 NSCLC患者中PD-L1表达与肿瘤分期、EGFR和ALK基因突变相关,并且PD-L1阴性表达者相对于阳性表达者有更长的生存期.
Abstract
Objective To investigate the expression of PD-L1 protein in non-small cell lung cancer(NSCLC),and to analyze the correlation between PD-L1 and EGFR,ALK,KRAS and the prognosis of NSCLC.Methods 164 patients who were diagnosed as NSCLC from January 2017 to April 2022 at the Department of pathology of the first division hospital of Xinjiang production and Construction Corps were collected retrospectively.The mutation status of EGFR,ALK and KRAS genes were detected by next generation sequencing,PD-L1 expression was detected by immunohistochemistry.Analyzing the correlation and prognosis of PD-L1 expression with EGFR,KRAS,and ALK genes in NSCLC patients.Results The expression of PD-L1 was no statistically significant differences in age,sex,smoking history,race,histological type and TNM staging(P＞0.05).According to the correlation analysis,PD-L1 expression was negatively correlated with EGFR gene mutation(R=-0.251,P＜0.01),positively correlated with ALK gene mutation(R=0.232,P＜0.01),and not correlated with BRAF gene mutation(R=-0.036,P＞0.05).Through one-year survival analysis,67.9％of patients with PD-L1 expression were positive and 84.1％were negative.Kaplan Meier Survival Analysis:Patients in the PD-L1 positive expression group were lower than those in the negative expression group(x2=4.505,P＜0.05).Conclusion PD-L1 expression is associated with tumor staging,EGFR,and ALK gene mutations in NSCLC patients,and PD-L1 negative expression has a longer survival period compared to positive expression.
关键词
非小细胞肺癌/PD-L1/EGFR/KRAS/ALK/预后Key words
Non-small cell lung cancer/PD-L1/EGFR/KRAS/ALK/Prognosis引用本文复制引用
基金项目
第一师阿拉尔市科技计划项目(2021YL05)
出版年
2024
NETL
NSTL
万方数据