Effect of down-regulation of miR-155 on Claudin-1 in intestine tissue of mice with traumatic brain injury
Objective To investigate the effect of miR-155 on the expression of tight junction protein Claudin-1 in intestinal mu-cosa of mice after brain injury(TBI),and to analyze the diagnostic value and therapeutic direction of miR-155 on intestinal mucosal barrier dysfunction after TBI.Methods Thirty C57BL/6J mice were divided in-to miR-155 group and control group.The miR-155 group was given AAV-mmu-mir-155-5p inhibitor,and the control group was given the same volume of normal saline.After 24 hours of intervention,the mice were euthanized and serum and small intestine tissue were collected for relevant testing.Results The serum Dao level of miR-155 treatment group was lower than that of the control group,and miR-155 could reduce the degree of intestinal in-jury,and the difference was statistically significant(P＜0.001);Pathological scores of small intestine tissues were performed under light microscope,compared with miR-155,the Chiu,s score of the control group was significantly lower(P＜0.001);Immunohistochemi-cal results showed that the expression of Claudin-1 in miR-155 group was significantly increased and evenly distributed in the intestinal epithelial mucosa.The expression of Claudin-1 in the control group was significantly lower than that in miR-155 group,and showed a patchy distribution,the expression difference between the two groups was statistically significant(P＜0.001).There was no significant difference in the expression of occludin in intestinal mucosal tissue between the two groups(P＞0.05).Conclusions miR-155 plays an important role in the development of intestinal mucosal barrier dysfunction,and it may affect the intestinal mucosal barrier function by targeting claudin-1.
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