The protective effect of SIRT muscle injury and mitochondrial autophagy based on a rat model of muscular atrophy induced by sepsis
Objective To investigate the protective effect of SIRT on myocardial injury and the effect of mitochondrial autophagy on myocardial cells by establishing a rat model of muscular atrophy induced by sepsis.Methods Sixty-five male SD rats with a weight of 200g and 300g were randomly divided into group A and group B,and then a rat model of muscular atrophy induced sepsis was established.The modeling of TNF-α and IL-6 levels was successfully detected by ELISA method.Mitochondrial membrane poten-tial,ATP and reactive oxygen species were detected to evaluate their functions.Western blot was used to detect the changes of SIRT,PINK1,FOXO3a,Parkin,and LC3-Ⅱ/Ⅰ proteins.Results The levels of TNF-α and IL-6 in LPS group,model group,LPS+brake+SIRT group were higher than those in normal group and simple brake group(P＜0.01).Compared with the model group,the levels of TNF-αand IL-6 in LPS+brake+SIRT group were decreased(P＜0.01).The levels of MMP and ATP in the model group were lower than those in the normal group,LPS group,and simple brake group,while ROS levels were increased(P＜0.01).The protein levels of SIRT,FOXO3a,PINK1,Parkin,and LC3-Ⅱ/Ⅰ in the model group were lower than those in normal group,LPS group and simple brake group(P＜0.01).The level of mitochondrial protein in group with LPS combined with immobilization and mitochondrial au-tophagy agonist berberine was higher than that in LPS+immobilization+SIRT group(P＜0.01).Conclusion During the process of sepsis induced muscular atrophy,the levels of SIRT and mitochondrial autophagy are reduced,and mitochondrial function is damaged.Intervention with SIRT can improve the progress of muscle atrophy caused by sepsis.
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