To explore the effect of metformin on bleomycin-induced pulmonary fibrosis in rats
Objective To investigate the effect of metformin on bleomycin-induced pulmonary fibrosis in rats,and to explore the changes of AMPK,NF-κB and TGF-β signalling factors in lung tissue.Methods Rats were randomly divided into blank group,model group,metformin group,prednisone group and pirfenidone group.Normal saline was injected into the trachea of rats as blank control group.The remaining rats were injected bleomycin into the trachea for a single time to establish pulmonary fibrosis model,and were treated with different drugs after 14 days:the rats were sacrificed after 14 days of continuous administration of normal saline,metfor-min,prednisone and pirfenidone.The lung tissues were stained with HE and Masson staining,and the scores of alveolar inflammation and pulmonary fibrosis in each group were compared.The levels of TGF-β,NF-κB and AMPK in lung tissue homogenate and alveolar lavage fluid were detected by enzyme-linked immunosorbent assay.Results HE staining showed that the inflammatory score of model group was higher than that of control group,metformin group,pirfenidone group,and prednisone group was higher than that of metfor-min group.The Masson staining scores in the metformin and pirfenidone groups were lower than those in the model group.The content of AMPK in BALF in the metformin group was higher than that in the other groups.The expression of NF-κB in the model group was higher than that in the metformin group,prednisone group and pirfenidone group,and the expression in the metformin group was higher than that in the prednisone group and pirfenidone group.The content of TGF-β in lung tissue was higher in the model group than in the other groups.Conclusion Metformin can alleviate the degree of pulmonary fibrosis induced by bleomycin in rats,which may be pro-duced by affecting the expression of TGF-β,NF-κB and AMPK signalling factors.
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