MiR-29c downregulated and regulated cell proliferation and apoptosis by suppression S100A4 in human gastric carcinoma cell lines
Objective To explore the effect of MicroRNA-29c(miR29c)on the proliferation and apoptosis of human gastric adeno-carcinoma cell lines,and to explore the relationship between miR29c and S100A4 metastasis-related protein.Methods The human gastric carcinoma cell lines BGC-823,SGC-7901 and normal gastric mucosa epithelial GES-1 cell line were cultured in carbon diox-ide incubator.The expreesion of miR29c in cell lines were tested by qRT-PCR method.BGC-823 cells were transfected with hsa-miR29c-mimics analogs(miR29c group)and hsa-miR29c-inhibitor(negative control group),respectively.In the control group,trans-fer solution was added,cell proliferation was detected by CCK8 and MTT,cell apoptosis was detected by flow cytometry.The expres-sion of S100A4-associated tumor-associated protein was detected by Western Blot.Results Compared with the expression rate of GES-1 in normal gastric mucosa epithelial cells(1.131±0.069),the expression rate of MiR-29c in gastric cancer cells BGC823(0.254±0.025),SGC-7901(0.466±0.057)(F=57.5,p<0.05).The expression of MiR-29c in BGC823 cells transfected with miR29c mimics was 4.126±0.231,0.405±0.174,0.557±0.102,and the differences were statistically significant(F=850.1,p<0.05).After transfection for 24h,48h and 72h,the OD450 values of miR29c group cells were 1.074±0.127,1.609±0.178 and 2.008±0.104,respectively.The negative control group was 0.419±0.169,0.477±0.092,0.738±0.153,and the control group was 0.830±0.305.After transfection for 48h and 72h,MiR-29c group was significantly higher than control group(F=59.46,p<0.05).After transfection for 24h,the apoptosis rates of miR29c group were(6.900±0.414)%,(4.490±0.316)%,(3.850±0.243)%,respectively.The apoptosis rates of the negative control group were(10.830±0.231)%,(11.940±0.279)%and(13.530±0.941)%,respectively.The apoptosis rates of the blank control group were:(10.190±0.188)%,(11.76±0.569)%,(13.260±0.987),(p<0.001).The expression of S100A4 protein in each group was 0.770±0.028,1.109±0.083,1.024±0.042,respectively(p=0.0021).Conclusion MiR29c can inhibit proliferation and induced apoptosis in hu-man gastric carcinoma cell lines through suppress S100A4 protein expression.
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