首页|药用植物紫草AP2/ERF基因家族生物信息学鉴定

药用植物紫草AP2/ERF基因家族生物信息学鉴定

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目的 紫草是重要的药用植物资源,鉴定研究紫草AP2/ERF转录因子家族,可靶定调控活性物质合成及代谢关键基因.方法 本研究基于紫草基因组,对紫草AP2/ERF转录因子家族进行保守结构域鉴定,理化性质预测,系统发育进化树构建,启动子区顺式作用元件分析.结果 紫草AP2/ERF转录因子家族共有177个成员,由AP2、RAV、ERF、DREB、Soloist5个亚家族组成;其分子量在9.0~73.0 kDa之间,等电点变化范围在4.0~11.0之间,主要定位在细胞核;LeERF与LeDREB亚家族细分为10个亚组,其中LeDREB亚家族缺少A3成员,LeERF亚家族缺少B2成员;其激素响应元件、胁迫响应元件、生长发育相关元件、光响应元件占主要分量.结论 本研究丰富了药用植物紫草的基因资源,为紫草地育种与深度开发奠定了理论基础.
Genome-wide Identification of AP2/ERF Gene Family in Lithospermum erythrorhizon
Objective Lithospermum erythrorhizon is an important medicinal plant resource,and the family of AP2/ERF transcrip-tion factors of L.erythrorhizon can be identified and studied,which can target key genes that regulate the synthesis and metabolism of active substances.Methods In this study,based on the genome of L.erythrorhizon,the AP2/ERF transcription factor family of L.eryth-rorhizon was identified using conserved domain analysis,and physicochemical property,phylogenetic relationships and cis-acting ele-ment were predicted and analyzed.Results There were 177 members of the AP2/ERF transcription factor family of L.erythrorhizon,which were composed of five subfamilies:AP2,RAV,ERF,DREB and Soloist.Their molecular weights ranged from 9.0 to 73.0 kDa,and their isoelectric points varied from 4.0 to 11.0,which were mainly localized in the nucleus.The LeERF and LeDREB subfamilies were subdivided into 10 subgroups,of which LeDREB subfamilies lacked A3 member and LeERF subfamily lacked B2 member.The hormone-response elements,stress-response elements,growth and development-related elements,and light response elements com-prise the main components.Conclusion This study enriches the genetic resources of the medicinal plant,and laid a theoretical founda-tion for the breeding and in-depth development of L.erythrorhizon.

Lithospermum erythrorhizonAP2/ERFTranscription factorsGenomeMetabolites

王静、雷慧兰、刘晓洁、蔡阳

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新疆维吾尔自治区药品检验研究院,乌鲁木齐 830054

中国科学院新疆生态与地理研究所荒漠植物保育及抗逆分子机理团队,乌鲁木齐 830011

新疆抗逆植物基因资源保育与利用重点实验室,乌鲁木齐 830011

紫草 AP2/ERF 转录因子 基因组 代谢物

2024

新疆医学
新疆维吾尔自治区医学会

新疆医学

影响因子:0.385
ISSN:1001-5183
年,卷(期):2024.54(8)