Extraction and separation of iridoid glycosides and lignans from Siberian Sambucus Nakai
Objective To extract and isolate iridoid glycosides and lignans from Siberian Sambucus Nakai,and to establish a meth-od for the determination of monosin and total lignans in Siberian Sambucus Nakai.Methods The effective components were extracted by aqueous alcohol and adsorbed by macroporous resin.The content of monosin in Sambucus Nakai was determined by HPLC and the content of total lignans in elderberry was determined by UV spectrophotometry.Results The active components of Sambucus Nakai were rich in iridoid glycosides,lignans and triterpenes.The contents of monoside in Sambucus Nakai extract and 30%ethanol elution were 0.254%and 2.63%,respectively.The total lignan content of 50%elution site was 38.07%.Conclusion The content of active com-ponents inSambucus Nakai was determined by HPLC and UV spectrophotometry,in order to provide a certain scientific basis for the development and utilization of Sambucus Nakai.
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