A method of ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)was established to rapidly and accurately determine the content of azelaic acid in complex sample matrix.The calibration curve of azelaic acid showed good linearity(R2=0.994 5)in the detection range of 0.05-20 mg·L-1.The limit of detection was 0.15 mg·kg-1,and the limit of quantitation was 0.5 mg·kg-1.The spiked recoveries of azelonic acid ranged from 81.9%to 105.4%with RSD of 2.2%-7.5%in cosmetic matrix(gel and cream)and transdermal test matrix(extracting solution from skin and receptor solution under skin)(n=6).This method has advantages such as simple pretreatment no need for previous derivatization,specificity and high sensitivity.It is suitable for the determination of azelaic acid in complex sample matrix.
关键词
化妆品/壬二酸/复杂样品体系/超高效液相色谱-三重四级杆串联质谱
Key words
cosmetics/azelaic acid/complex sample matrix/ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)
维普
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