Determination of Azelaic Acid by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry
A method of ultra high performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)was established to rapidly and accurately determine the content of azelaic acid in complex sample matrix.The calibration curve of azelaic acid showed good linearity(R2=0.994 5)in the detection range of 0.05-20 mg·L-1.The limit of detection was 0.15 mg·kg-1,and the limit of quantitation was 0.5 mg·kg-1.The spiked recoveries of azelonic acid ranged from 81.9%to 105.4%with RSD of 2.2%-7.5%in cosmetic matrix(gel and cream)and transdermal test matrix(extracting solution from skin and receptor solution under skin)(n=6).This method has advantages such as simple pretreatment no need for previous derivatization,specificity and high sensitivity.It is suitable for the determination of azelaic acid in complex sample matrix.
cosmeticsazelaic acidcomplex sample matrixultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)
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维普
