Simultaneous Determination of Nine Catechins and Three Alkaloids in Tea Extracts by HPLC
A high-performance liquid chromatography(HPLC)method had been developed using a Kromasil 100-5-C18 analytical column and a gradient elution with methanol and 0.01%(in volume fraction)phosphoric acid water as the mobile phase.This method allowed for simultaneous quantification of 12 compounds,including 9 catechins and 3 alkaloids,in tea extracts.The results demonstrated a good linear relationship between peak area and concentration within a certain range of mass concentration.The method had a quantification limit of 2.5-25 mg/L,with a recovery rate of 94%-103%and the relative standard deviations(RSDs)were≤2.7%.This method offers high precision,accuracy,short analysis time,and efficient detection,making it a reliable approach for studying tea extracts and suitable for quality control of tea raw materials.
万方数据
维普
