三七总皂苷和马齿苋多糖合用的抗炎功效评价
Anti-Inflammatory Effect of Panax Notoginseng Saponins Combined with Portulaca Oleracea Polysaccharide
夏金凤 1陈影 2杨万松 3张钰雯 1陈真1
作者信息
- 1. 昆明医科大学科技成果孵化中心,云南昆明 650500
- 2. 昆明医科大学人文与管理学院,云南昆明 650500
- 3. 云南悦芳季生物资源开发有限公司,云南昆明 650501
- 折叠
摘要
为了探讨不同质量浓度下三七总皂苷和马齿苋多糖的协同抗炎组方,将不同质量浓度的三七总皂苷(1、5、25、125 μg/mL)和马齿苋多糖(2、10、50、250 μg/mL)两两复配,以 100 μg/mL地塞米松为阳性对照,通过测定炎症因子TNF-α的抑制率评估 16 种复配组合物的抗炎功效,并采用协同分数(synergy score值)系统定量评价复配组合物中 2 种成分的相互作用.结果显示,当质量浓度为 125 μg/mL的三七总皂苷与 50 μg/mL或 250 μg/mL的马齿苋多糖复配时,均对TNF-α具有显著的抑制效应,抑制率分别为45.18%和 61.42%,其抑制作用与 100 μg/mL的地塞米松(抑制率为53.17%)无显著性差异(P>0.05),synergy score值分别为 15.58 和 26.44.综上,125 μg/mL的三七总皂苷与 50 μg/mL或 250 μg/mL的马齿苋多糖合用时协同抑制TNF-α的产生,但其余 14 种更低剂量的复配组合仅表现为相加作用.
Abstract
To investigate the synergistic anti-inflammatory effects of Panax notoginseng saponins(PNS)and Portulaca oleracea polysaccharide(POP)at different dosage concentrations.PNS(1,5,25,125 μg/mL)and POP(2,10,50,250 μg/mL)were respectively combined in pairs at different concentrations,and 100 μg/mL dexamethasone was used as a positive control.The inhibition rate of TNF-α of 16 combinations was studied,and the interaction of the two components was quantitatively evaluated by the synergy score system.PNS at 125 μg/mL combined with POP at 50 μg/mL or 250 μg/mL effectively suppressed TNF-α production,and the inhibitory rates were 45.18%and 61.42%respectively.The inhibitory effect was not significantly different from that of dexamethasone 100 μg/mL(53.17%)(P>0.05).Synergy scores were 15.58 and 26.44,respectively.It was suggested that 125 μg/mL PNS combined with 50 μg/mL or 250 μg/mL POP could synergically inhibit the production of TNF-α,but the other 14 lower dose combinations only showed the additive effect.
关键词
协同作用/三七总皂苷/马齿苋多糖/炎症因子Key words
synergistic effect/Panax notoginseng saponins/Portulaca oleracea polysaccharide/inflammatory factor引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据