摘要
建立了高效液相色谱法同时检测化妆品中 8 种非甾体抗炎药物成分的方法.样品经体积比 8:2 的甲醇-水(含质量分数 0.2%乙酸)溶液超声提取,滤过,采用 20 mmol/L乙酸铵溶液(含质量分数 0.2%乙酸)-甲醇为流动相进行梯度洗脱,检测波长为230 nm,通过SunFire C18 柱(250 mm×4.6 mm×5 μm)色谱柱进行分离.结果表明,8种成分在各自的质量浓度范围内线性关系良好,相关系数(R2)均大于 0.999 5,检出限为 0.5～10.2 mg/kg,在 3 个不同添加水平下,平均回收率为90.9%～108.2%,相对标准偏差均小于5%.该方法样品前处理简单、快速,适用于化妆品中多种非甾体抗炎药物成分的同时检测.
Abstract
A high performance liquid chromatographic(HPLC)method was developed for the simultaneous determination of 8 non-steroidal anti-inflammatory drug components in cosmetics.The samples were ultrasonically extracted by a mixed solution of methanol-water(containing 0.2%acetic acid in mass fraction)at the ratio of 8:2 in volume,then filtered.Separation was conducted on a SunFire C18 column(250 mm×4.6 mm×5 μm)with gradient elution of 20 mmol/L ammonium acetate(containing 0.2%acetic acid in mass fraction)and methanol,and the detection wavelength was 230 nm.The results showed that the linearity of the 8 components was good in their respective mass concentration ranges with the correlation coefficients(R2)greater than 0.999 5,and the limits of detection ranged from 0.5 to 10.2 mg/kg.The average recoveries of the 8 components ranged from 90.9%to 108.2%at 3 different spiked levels with the relative standard deviations less than 5%.The method has simple and rapid sample pretreatment,suitable for the simultaneous determination of various non-steroidal anti-inflammatory drug components in cosmetics.
NETL
NSTL
维普
万方数据