Simultaneous Determination of 17 Preservatives and Antioxidants in Toothpaste by HPLC
A method for the simultaneous determination of 17 preservatives and antioxidants in toothpaste by high performance liquid chromatography(HPLC)was established.The sample was extracted by methanol,separated by Capcell Pak C18 MGⅡcolumn,eluted with acetonitrile-methanol-0.02 mol/L ammonium acetate solution,and detected at 280 nm by HPLC-diode array detector(DAD).The external standard method was used to quantify the content.The results showed a good linearity in the range of 4.43-542.61 μg/mL with the correlation coefficients(R2)of 0.997 75-0.999 96.The recovery rates were 81.7%-116.7%,the relative standard deviations(RSDs)were no more than 2.9%,and the within-day precisions were 0.3%-2.1%.The limits of detection were in the range of 0.2-5.1 μg/g,and the limits of quantitation were in the range of 0.5-17 μg/g.The method is sensitive,accurate,fast,stable,and meets the daily inspection needs of 17 preservatives and antioxidants in toothpaste.
NETL
NSTL
万方数据
维普
