Establishment and Application of U937 Cell Line Activation Assay Method
U937 cell line activation assay(U-SENSTM)as an animal alternative method was established to evaluate the feasibility for skin sensitization of cosmetic ingredients.Referring to the experimental process of U-SENSTM in OECD TG 442E,16 chemical substances were tested and compared with the blind sample testing results of 8 reference substances in 4 laboratories.The results showed that 15 test substances were consistent with the results from LLNA,with an overall accuracy of 100%(15/15)compared with LLNA and 85%(11/13)compared with human data.The accuracy of detection results of 8 reference substances from 4 laboratories were 100%accurate.The U-SENS TM method was established successfully to provide a reference for screening the skin allergens.
cosmeticsanimal alternative methodU-SENS™skin sensitization
NETL
NSTL
万方数据
