U937细胞激活试验方法的建立及应用
Establishment and Application of U937 Cell Line Activation Assay Method
所雅琼 1张凤兰 2罗飞亚 2裴新荣 2邢书霞 2王钢力2
作者信息
- 1. 北京市利康医院,北京 102600
- 2. 中国食品药品检定研究院,北京 100050
- 折叠
摘要
建立U937 细胞系激活试验(U-SENSTM)方法,并评价该方法作为体外替代方法用于评估化妆品原料的皮肤致敏性检测的可行性.参照经合组织(OECD)化学品检测指南的TG 442E中U-SENSTM试验流程,对 16 种化学物质进行检测,并在 4 个实验室进行 8 种参考物质的盲样检测.结果显示,其中 15 种受试物检测结果与小鼠局部淋巴结试验(LLNA)方法的结果均一致,与LLNA相比总体准确性为 100%(15/15),与人体数据相比总体准确性为 85%(11/13);4个实验室对8种OECD参考物质检测的准确率均为100%.成功建立了U-SENSTM试验方法,为筛选皮肤致敏原提供参考.
Abstract
U937 cell line activation assay(U-SENSTM)as an animal alternative method was established to evaluate the feasibility for skin sensitization of cosmetic ingredients.Referring to the experimental process of U-SENSTM in OECD TG 442E,16 chemical substances were tested and compared with the blind sample testing results of 8 reference substances in 4 laboratories.The results showed that 15 test substances were consistent with the results from LLNA,with an overall accuracy of 100%(15/15)compared with LLNA and 85%(11/13)compared with human data.The accuracy of detection results of 8 reference substances from 4 laboratories were 100%accurate.The U-SENS TM method was established successfully to provide a reference for screening the skin allergens.
关键词
化妆品/动物替代方法/U-SENS™/皮肤致敏Key words
cosmetics/animal alternative method/U-SENS™/skin sensitization引用本文复制引用
出版年
2024
NETL
NSTL
万方数据