Combination Application of In Vitro Evaluation Methods for Cosmetic Efficacy
Based on the properties and efficacy of the main ingredients in cosmetics,a multidimensional in vitro efficacy evaluation was conducted using biochemical method,in vitro cell model,and in vitro recombinant 3D epidermal model to evaluate firming,anti-wrinkle,and moisturizing effects.The skin care essence showed good antioxidant and oxidative stress damage protection effects.The mass fraction of 1.25%skin care essence solution can effectively save the damaged proliferation and vitality of human skin fibroblasts(HSF)cells inhibited by UVA radiation.Compared with the model group,the content of skin care essence solution not less than 1.25%(in mass fraction)can significantly increase the content of Col-I and reduce the content of MMP-1.It showed that the skin care essence had obvious effect of firming and anti-wrinkle.Comparing with the blank control group,the pyrrolidone carboxylate(PCA)content in the cuticle of the in vitro recombinant 3D epidermal models after the action of skin care essence(1.25%and 2.5%,in mass fraction)increased significantly.Above all,the skin care essence showed moisturizing efficacy.
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