Simultaneous Determination of Ten Preservatives in Shampoo by HPLC
A method for simultaneous determination of 10 preservatives such as phenol in shampoo by high performance liquid chromatography(HPLC)was established.After methanol extraction,the samples were eluted by gradient elution on a Capcellpak C18 MG Ⅲ column(250 mm×4.6 mm×5 μm)with acetonitrile,methanol and 0.02 mol/L ammonium acetate solution as mobile phases.A diode array detector was used to detect 10 preservatives in shampoo at 280 nm.In the range of 5-100 μg/mL,the mass concentration of 10 preservatives had a good linear relationship with the peak area(r2≥0.998).The limits of detection(LOD)were 0.4-2.2 μg/g,the limits of quantification(LOQ)were 1.2-7.3 μg/g,the average recoveries were 85.2%-107.4%,and the relative standard deviation(RSD)was 0.7%-4.8%(n=6).The results showed that the method was simple,sensitive,accurate and repeatable,and could be used for simultaneous determination of various preservatives in shampoo.
high performance liquid chromatographyshampoopreservative
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