Preparation of Gardenia Jasminoides Fruit Oil and Its Soothing and Repairing Efficacies
The fatty acid composition of Gardenia jasminoides fruit oil(ZGY)with preparation by supercritical CO2 technology was analyzed through GC-MS.The cell viability was detected by CCK8 method,ELISA and qPCR were applied to detect different key indexes under cell injury.The results showed that the fatty acids of ZGY included 44.85%linoleic acid,36.26%oleic acid,15.17%palmitic acid and 82.34%unsaturated fatty acids.Compared with the model group,ZGY had a strong inhibitory on NO production in LPS-induced RAW 264.7 cells.After simultaneous treatment with 0.625%and 1.25%ZGY in volume fraction,it significantly inhibited the expression levels of IL-6 and IL-8 mRNA,and increased the expression levels of LOR,ZO-1 and CLDN3 mRNA in UVB irradiation-HaCaT cells.Therefore,ZGY can reduce the production of NO,down-regulate the expression of inflammatory factor mRNA,up-regulate the expression of barrier factor mRNA,and play a soothing and repairing role,which provides a theoretical basis for the application and development of ZGY.
Gardenia jasminoides fruit oilsoothingrepairingfatty acidUVB
万方数据
维普
