Streptococcus egui subsp.zooepidemicus was used as the source of hyaluronic acid enzyme fermentation subspecies,high-purity hyaluronic acid disaccharide was obtained through the specific degradation of hyaluronic acid enzyme and organic ultrafilm filtration of hyaluronic acid.Its molecular weight was 400 Da by high performance liquid chromatography(HPLC)method.Taking the successful preparation of hyaluronic acid disaccharide as the research object,the detection showed that the gene expressions of type Ⅲ and type ⅩⅦ collagen in fibroblasts were significantly up-regulated,the contents of type Ⅰcollagen and elastin were significantly increased,and the synthesis of AQP3 in keratinocytes was improved.The expressions of Caspase-14,FLG,LOR,CD44,Has1 and Has3 protein genes were significantly up-regulated.It's a new data basis for hyaluronic acid disaccharide as a cosmetic raw material.
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