Evaluation of sensitization of multicomponent compound preservatives by direct peptide reaction assay(DPRA),amino acid derivatization reaction assay(APRA)and local closure patch test(BT).The DPRA method measured 56.25%and 62.35%peptide depletion for propylparaben+methylisothiazolinone and imidazolidinylurea+methylisothiazolinone combinations,respectively,with strong positive predictions,and 32.55%peptide depletion for propylparaben+imidazolidinylurea combination,which predicted weak positive results,which was consistent with the results of APRA and BT methods.The DPRA method and the APRA method could be effectively used for the 3 types of combinations,namely,non-sensitizing plus weakly sensitizing,non-sensitizing plus strongly sensitizing,and weakly sensitizing plus strongly sensitizing sensitization evaluation;the strong sensitizer component in multicomponent complex preservatives is the main determinant of the depletion of peptide and amino acid derivatives.The in vitro and in vivo combination method can be effectively used for the preliminary evaluation of the sensitization of multicomponent compound preservatives.
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