Characterization of Coenzyme Q and Preliminary Investigation on Its Effect on Life-span in Drosophila melanogaster
Coenzyme Q is an essential component of the mitochondrial respiratory chain as a lipid-soluble electron transporter located in the inner membrane of mitochondria. The number of isoprenoid subunits in the coenzyme Q side chain varies in different species. The content of coenzyme Q in Drosophila has not been reported yet. In the present report , we analyzed the content of coenzyme Q in adult Drosophila by HPLC. We provided evidence that Drosophila contains both CoQ9 and CoQ10. The amount of CoQ9 is about 2. 5 folds than that of CoQ10. We further showed the content of CoQ9 and CoQ10 in females are respectively about 30 ％ , 33 ％higher than that in males. In addition,both concentrations of CoQ9 and CoQ10 are significanrly reduced in the mutant of CoQ synthase gene coqZ which appeared with shorrened life-span. These results provided a good basis to further invesrigate in vivo biological functions of CoQ in Drosophila.
国家科技期刊平台
NSTL
万方数据
维普
