Objective To study the sensitization of Reduning injection and the contained chlorogenic acid to RBL-2H3 cells and animals. Methods The content of histamine released from RBL-2H3 cells under the stimulation of single dose of Reduning injection and the contained chlorogenic acid was determined. After RBL-2H3 cells were pretreated by specific IgE rat serum induced by antigen such as Reduning injection and the contained chlorogenic acid, the content of histamine released from the cells added with Reduning injection and the contained chlorogenic acid was determined again; active systemic Anaphylaxis(ASA) experiment on guinea pigs and passive cutaneous anaphylaxis (PCA) experiment on rats were made in order to observe the in-vivo sensitization of Reduning injection and the contained chlorogenic acid. and the contained chlorogenic acid was determined. After RBL-2H3 cells were pretreated by specific IgE rat serum induced by antigen such as Reduning injection and the contained chlorogenic acid, the content of histamine released from the cells added with Reduning injection and the contained chlorogenic acid was determined again; active systemic Anaphylaxis(ASA) experiment on guinea pigs and passive cutaneous anaphylaxis (PCA) experiment on rats were made in order to observe the in-vivo sensitization of Reduning injection and the contained chlorogenic acid.Results Single dose of Reduning injection and chlorogenic acid did not induce RBL-2H3 cells to release histamine; after RBL-2H3 cells were pretreated by specific IgE rat serum, Reduning injection and the contained chlorogenic acid were used for induction again, but the released amount of histamine had no significant changes; ASA and PCA animal experiments results showed that Reduning injection and the contained chlorogenic acid were negative. Conclusion Clinicaldose of Reduning injection and the contained chlorogenic acid will not induce any allergic reaction of rats and guinea pigs.
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