首页|牛角瓜组培快繁过程中生理生化指标的变化

牛角瓜组培快繁过程中生理生化指标的变化

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以牛角瓜组培苗为材料,对牛角瓜组培快繁过程中不同阶段的几种生理生化指标进行了测定,为其组培器官发生的调控提供依据。结果表明:牛角瓜增殖和生根过程中的形态变化与SOD、 POD、 CAT活性、可溶性糖和可溶性蛋白含量存在一定的相关性;在增殖过程中SOD活性变化先升后降, POD活性变化规律总体呈现下降趋势, CAT的活性变化呈现上升趋势,可溶性蛋白含量变化先降后升,可溶性糖含量呈上升趋势。在生根过程中SOD、 POD和 CAT酶的活性变化趋势基本一致,总体呈现“上升—下降—上升”,可溶性蛋白含量总体变化趋势为“升高—下降—缓慢升高”,可溶性糖含量总体变化趋势为先缓慢升高,后又缓慢下降。
Changes of P hysiological and Biochemical Index of Calotropis gigantea in the P rocess of Tissue Culture and Rapid P ropagation
Taking tissue culture seedling of Calotropis gigantea as the material, some physiological-biochemical index were studied during different growth periods in the process of tissue culture and rapid propagation, and it pro-vided the basis for the regulation of the morphology of plant′s organs. The results were as follows:There were some relationships between the morphosis and the activity of SOD, POD, CAT and the content of soluble sugar and solu-ble protein in the stage of proliferation and rooting. During the proliferation stage, SOD activity was first increased and then decreased, and POD activity showed a declining tendency as a whole, and CAT activity showed an in-creasing tendency, and the content of soluble protein was firstly decreased and then increased, and the content of soluble sugar was increased. During the rooting stage, activity of SOD, POD and CAT showed a same tendency of“rise-fall-rise”, and the content of soluble protein was firstly increased, then decreased and finally increased slow-ly, and the content of soluble sugar was firstly increased slowly and then decreased slowly.

Calotropis giganteaanti-oxidant enzyme activitysoluble proteinsoluble sugartissue culture and rapid propagation

唐军荣、郑元、刘惠民、马焕成、陈诗、刘忠美

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西南林业大学西南地区生物多样性保育国家林业局重点实验室,云南 昆明650224

西南林业大学云南省高校林木遗传改良与繁育重点实验室,云南 昆明650224

牛角瓜 抗氧化酶活性 可溶性蛋白 可溶性糖 组培快繁

国家林业局林业公益性行业项目西南林业大学云南省省级重点学科(林学)西南地区生物多样性保育国家林业局重点实验室开放基金

201304810

2016

西南林业大学学报
西南林业大学

西南林业大学学报

CSTPCD北大核心
影响因子:0.773
ISSN:2095-1914
年,卷(期):2016.36(3)
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